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Rattus norvegicus

ABSTRACT: DNA topoisomerase IIbeta (topo IIbeta) regulates gene expression in developing cerebellar granule cells (CGCs)

PROVIDER: PRJDB3097 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR023138_1.fastq.gz	Fastqsanger.gz
	DRR023138_2.fastq.gz	Fastqsanger.gz
	DRR023139_1.fastq.gz	Fastqsanger.gz
	DRR023139_2.fastq.gz	Fastqsanger.gz
	DRR023140_1.fastq.gz	Fastqsanger.gz

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Project description:DNA topoisomerase IIbeta (topo IIbeta) regulates gene expression in developing cerebellar granule cells (CGC)

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Project description:DNA topoisomerase II (topo II) catalyzes a strand passage reaction in that one duplex is passed through a transient brake in another. Completion of late stages of neuronal development depends on the presence of active isoform (topo IIbeta). We identified topo IIbeta action sites on 7 selected genomic regions (about 79 Mb in total), each containing at least one gene that is controlled by topo IIbeta in neuronal differentiation. We used a novel method, etoposide-mediated topoisomerase immunoprecipitation (eTIP), followed by identification of precipitated DNA fragments on genomic tiling arrays. These DNA fragments were first fractionated by concentrated salt prior to the array analysis. The 0.5 M NaCl-released fraction was analyzed as P2 and the salt-resistant fraction wad analyzed as P1.

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