Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drosophila melanogaster

ABSTRACT: RNA-seq analysis of long-term olfactory aversive memory in the mushroom body of Drosophila melanogaster.

PROVIDER: PRJDB3400 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR029950.fastq.gz	Fastqsanger.gz
	DRR029951.fastq.gz	Fastqsanger.gz
	DRR029952.fastq.gz	Fastqsanger.gz
	DRR029953.fastq.gz	Fastqsanger.gz
	DRR029954.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Transcriptome Analysis of Drosophila Mushroom Body Neurons by Cell Type Reveals Memory-Related Changes in Gene Expression

Project description:We report the application of low cell number sequencing of identifiable Drosophila melanogaster neurons following behavior. We demonstate the feasibility of identifying the transcriptome of 5 Mushroom Body output Neurons and 2 classes of Kenyon Cells. We find these neurons display a diverse repertoire of receptors and signaling transcripts. This information alone seems to be enough to identify each class of neurons in the study. In additional we show that aversive long-term memory induces changes in gene transcript levels in a subset of these neurons. This study provides a framework for identifying neuronal classes in Drosophila melanogaster and gaining insight into the interplay between behavior and gene regulation.

2016-05-05 | GSE74989 | GEO

Regions of accessible chromatin in adult mushroom body neurons of Drosophila melanogaster [Trx_ATAC]

Project description:We have analyzed regions of accessible chromatin in adult mushroom body neurons of Drosophila melanogaster of a control genetic background using ATAC-seq.

2025-01-14 | GSE273839 | GEO

Memory phase-specific genes identified by CrebB-target DamID in the Mushroom Bodies

Project description:The formation of long-term memories requires changes in the transcriptional program and de novo protein synthesis. One of the critical regulators for long-term memory (LTM) formation and maintenance is the transcription factor CREB. Genetic studies have dissected the requirement of CREB activity within memory circuits, however less is known about the genetic mechanisms acting downstream of CREB and how they may contribute defining LTM phases. To better understand the downstream mechanisms, we here used a targeted DamID approach (TaDa). We generated a CREB-Dam fusion protein using the fruit fly Drosophila melanogaster as model. Expressing CREB-Dam in the mushroom bodies (MBs), a brain center implicated in olfactory memory formation, we identified genes that are differentially expressed between paired and unpaired appetitive training paradigm. Of those genes we selected candidates for an RNAi screen in which we identified genes causing increased or decreased LTM.

2022-10-19 | GSE216045 | GEO

Blobby is a synaptic active zone assembly protein required for memory in Drosophila

Project description:At presynaptic active zones (AZs), scaffold proteins are critical for coordinating synaptic vesicle (SV) release and forming essential nanoarchitectures. However, regulatory principles steering AZ scaffold assembly, function, and plasticity remain insufficiently understood. We here identify a novel Drosophila AZ protein, "Blobby", essential for proper AZ nano-organization. Blobby biochemically associates with the ELKS family AZ scaffold protein Bruchpilot (BRP) and integrates into newly forming AZs. Loss of Blobby results in fewer AZs forming, ectopic AZ scaffold protein accumulations ("blobs") and disrupts nanoscale architecture of the BRP-AZ scaffold. Functionally, blobby mutants show diminished evoked synaptic currents due to reduced SV release probability and fewer functional SV release sites. Blobby is also present in adult brain synapses, and post-developmental knockdown of Blobby in the mushroom body impairs olfactory aversive memory consolidation. Thus, our analysis identifies a new layer of AZ regulation critical for developmental AZ assembly but also for AZ-mediated plasticity controlling behavior.

2025-03-17 | PXD058345 | Pride

Mushroom body cell type comparison

Project description:The Drosophila melanogaster mushroom bodies (MBs) are brain structures critical for olfactory memory. The approximately 2000 intrinsic MB neurons are divisible into alpha/beta neurons, alpha'/beta' neurons and gamma neurons by morphology and roles in memory processing. It has also been shown that the different subtypes of MB neurons have different function in the process of memory formation. This difference in function, suggested to us that different types of MB neurons might have different expression profiles. In this study, we used cell-type specific gene expression profiling to gain insight into cellular properties of MB neurons.

2013-01-10 | E-MEXP-3798 | biostudies-arrayexpress

Transcription profiling of Drosophila melanogaster ablated mushroom bodies vs unablated MB to investigate the repertoire of MB genes that control initiation and maintenance of neural differentiation during learning and memory

Project description:Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. To get insights of the repertoire of MB genes that control initiation and maintenance of neural differentiation as well as the repertoire of neural factors that may have functions in the synaptic plasticity of MB neurons during learning and memory, we compared the transcript profiles between wild type and MB-ablated brains using a Drosophila whole-genome microarray. Newly hatched larvae were briefly administered with a DNA-synthesis inhibitor, hydroxyurea, and raised to adults, from which total brain RNA was analyzed. Experiment Overall Design: Two conditions analyzed: Control Brains and Musroom Body-ablated brains. Experiment Overall Design: Each condition was analyzed in triplicate.

2007-11-01 | E-GEOD-3379 | biostudies-arrayexpress

Drosophila melanogaster Sleep deprivation

Project description:HPLC MS/MS confirmation of lipids found to be heterogeneously distributed between rested and sleep-deprived Drosophila melanogaster brain samples by MALDI MSI. Data belongs to publication "Kv Channels Integrate Sleep Pressure in a Voltage-Gated Lipid Peroxidation Memory"

2023-04-21 | MSV000091767 | MassIVE

Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [RNA-seq Set1 & trr]

Project description:We have analyzed changes in histone H3 lysine 4 methylation (H3K4me) and gene expression resulting from trx knockdown (KD) in adult mushroom body neurons of Drosophila melanogaster.

2024-12-17 | GSE268425 | GEO

Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [ChIP-seq]

2024-12-17 | GSE239492 | GEO

Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [RNA-seq trx]

2024-12-17 | GSE239491 | GEO