Project description:Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia. Using sequencing by synthesis (SBS) of an RNA-Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were developed. When evaluated with 48 individuals, these markers showed genetic variations ranging from two to 15 alleles and expected heterozygosity ranging from 0 to 0.881. These markers will be useful for examining the genetic diversity and structure of remaining wild populations and improving the genetic status of ex situ populations.
| S-EPMC4542945 | biostudies-literature
Project description:Phylogeny, biogeography and ecological diversification of New Caledonian palms (Arecaceae)
Project description:Cold acclimation in conifers is a complex process, the timing and extent of which reflects local adaptation and varies widely along latitudinal gradients for many temperate and boreal tree species. In spite of their ecological and economic importance, little is known about the global changes in gene expression that accompany autumn cold acclimation in conifers. Using three populations of Sitka spruce (Picea sitchensis) spanning the species range, and a Picea cDNA microarray with 21,840 unique elements, we monitored within and among-population gene expression during the fall. Microarray data were validated for selected genes using real-time PCR. Similar numbers of genes were significantly two-fold upregulated (1,257) and downregulated (967) between late summer and early winter. Among those upregulated were dehydrins, pathogenesis-related/antifreeze genes, carbohydrate and lipid metabolism genes, and genes involved in signal transduction and transcriptional regulation. Among-population microarray hybridizations at early and late autumn time points revealed substantial variation in the autumn transcriptome, some of which may reflect local adaptation. Our results demonstrate the complexity of cold acclimation in conifers, highlight similarities and differences to cold tolerance in annual plants, and provide a solid foundation for functional and genetic studies of this important adaptive process in conifers. Keywords: Time course
2008-06-01 | GSE8370 | GEO
Project description:Corvus moneduloides (New Caledonian crow) genome, bCorMon1
Project description:Analysis of Paulownia tomentosa cambial tissues at gene expression level. The hypothesis tested in the present study was that miRNAs can regulate the development of the vascular cambium.The results provide new insights into the important regulatory functions of miRNAs in vascular cambium development and wood formation in conifers.