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Bombyx mori

ABSTRACT: Identification of the enzyme that trims the 3'-end of piRNAs in silkworms

PROVIDER: PRJDB4053 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR039989.fastq.gz	Fastqsanger.gz
	DRR039990.fastq.gz	Fastqsanger.gz
	DRR039991.fastq.gz	Fastqsanger.gz
	DRR039992.fastq.gz	Fastqsanger.gz
	DRR039993.fastq.gz	Fastqsanger.gz

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Similar Datasets

Bombyx mori

Project description:Analysis of PIWI-bound piRNAs in silkworms

| PRJDB5142 | ENA

Gene expression profiling of the fat body of Bombyx mori exposed to phoxim insecticide

Project description:Transcriptional characteristics of genes in the fat body of domestic silkworms after 24 h exposure to phoxim through whole-genome oligonucleotide microarray.

2016-01-01 | GSE60877 | GEO

Gene expression profiling of the fat body of Bombyx mori exposed to phoxim insecticide

Project description:Transcriptional characteristics of genes in the fat body of domestic silkworms after 24 h exposure to phoxim through whole-genome oligonucleotide microarray. Transcriptional profiling of fat body in the domestic silkworms comparing control untreated fat body with phoxim-treated fat body Transcription profiling experiments, phoxim-treated fat body (samples) were analyzed. Dual-channel experiments, with test samples labeled by Cy5 and control samples labeled by Cy3. Three Biological replicate. No dye-swaps.

2016-07-03 | E-GEOD-60877 | biostudies-arrayexpress

Bombyx mori

Project description:Hsp90 facilitates accurate loading of precursor piRNAs into PIWI proteins

| PRJDB2257 | ENA

Bombyx mori

Project description:Pheromones were initially identified in silkworms, which are now studied for silk quality improvement

| PRJNA10637 | ENA

High throughput sequencing of Siwi and BmAGO3 associated piRNAs from Bombyx mori BmN4 cells

Project description:In germ cells, piRNAs are amplified through the Ping-Pong cycle that depends on reciprocal Slicer-mediated target RNA cleavage by two PIWI members. A germ-specific DEAD-box protein Vasa is required for the process. However, Vasa’s function is poorly understood. Here, we show that target RNAs cleaved by a Bombyx mori (silkworm) PIWI, Siwi, remain to be bound with the protein upon cleavage, but are released in the presence of Vasa in B. mori (BmVasa) and ATP. Under normal conditions, BmVasa co-purifies with Siwi, but not with second B. mori PIWI BmAgo3. However, when BmVasa loses the ATP-binding and RNA-unwinding activities, BmVasa avidly associates with Siwi and BmAgo3, which contains transposon transcripts predominantly in sense orientation, the sources of BmAgo3-piRNAs. Without BmVasa, BmAgo3 is devoid of piRNAs. Thus, BmVasa actively releases target RNAs from Siwi, upon its cleavage, to urge BmAgo3-piRNA complex formation in the Ping-Pong cycle, enabling continuous supply of piRNAs in germ cells.

2014-12-31 | GSE58221 | GEO

Bombyx mori

Project description:The MID-PIWI module of Piwi proteins specifies nucleotide- and strand-biases of piRNAs

| PRJNA239665 | ENA

Bombyx mori

Project description:High throughput sequencing of Siwi and BmAGO3 associated piRNAs from Bombyx mori BmN4 cells

| PRJNA251635 | ENA

Bombyx mori

Project description:Single-Base Resolution Methylomes of Silkworms and Functional Importance of Gene Methylation in Insects Revealed by Ultra-High-Throughput Sequencing

| PRJNA118053 | ENA

RNA associating with PIWI proteins in Bombyx mori BmN4 cells

Project description:RNAs associating with PIWI proteins were Immunoisolated from BmN4 cells. Sequence libraries were generated with NEBNext Small RNA Library Prep Set for Illumina(NEB). Libraries were sequenced using Illumina MiSeq (single-end, 51 cycles).

2020-09-02 | GSE150444 | GEO

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