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Terrestrial metagenome

ABSTRACT: Diversity of Ktedonobacteria in terrestrial environments

PROVIDER: PRJDB5129 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR067880_1.fastq.gz	Fastqsanger.gz
	DRR067880_2.fastq.gz	Fastqsanger.gz
	DRR067881_1.fastq.gz	Fastqsanger.gz
	DRR067881_2.fastq.gz	Fastqsanger.gz
	DRR067882_1.fastq.gz	Fastqsanger.gz

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Project description:Marine is one of the most important resources of microorganisms, including bacteria, actinomycetes, and fungi. As marine and terrestrial environments differ a lot in many aspects it is not surprising that the species and characteristics of microorganisms living there are very different. Interestingly, many marine microorganisms can find their congeners of the same species from terrestrial resources. The aim of this work is to evaluate the intraspecies differences between marine and terrestrial actinomycetes on metabolic level and to uncover the mechanism responsible for the differences. To address this, we carried out comparative metabolomics study on Nesterenkonia flava strains isolated from marine and terrestrial environments. The results showed that marine strains were clearly distinguished from their terrestrial congeners on the principal components analysis (PCA) scores plot of intracellular metabolites. The markers responsible for the discrimination of marine and terrestrial strains were figured out using loading plot from partial least squares discrimination analysis (PLS-DA). Pathway analysis based on PLS-DA, univariate analysis, and correlation analysis of metabolites showed that the major differential metabolites between the terrestrial N. flava and the marine ones were involved in osmotic regulation, redox balancing, and energy metabolism. Together, these insights provide clues as to how the previous living environment of microbes affect their current metabolic performances under laboratory cultivation conditions.

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Project description:Characterisation of terrestrial dimethylsulfide-degrading bacteria using stable isotope probing

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