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Oryza longistaminata strain:IRGC110404

ABSTRACT: Genotyping-by-sequencing of an Oryza longistaminata x Oryza sativa F2 population.

PROVIDER: PRJDB5346 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR077434.fastq.gz	Fastqsanger.gz
	DRR077435.fastq.gz	Fastqsanger.gz
	DRR077436.fastq.gz	Fastqsanger.gz
	DRR077437.fastq.gz	Fastqsanger.gz
	DRR077438.fastq.gz	Fastqsanger.gz

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transcriptomics during pollen development in Oryza longistaminata

Project description:Oryza longistaminata is an African wild rice species that possesses special traits for breeding applications. Self-incompatibility is the main cause of sterility in O. longistaminata, but here we demonstrated that its pollen fertility and vitality are normal. Lipid and carbohydrate metabolism were active throughout pollen development. In this study, transcriptomics quantitative analysis was used to investigate the profiles of genes related to lipid and carbohydrate metabolism in 4-, 6- and 8.5-mm O. longistaminata spikelets before flowering. We documented cytological changes throughout important stages of anther development, including changes in reproductive cells as they formed mature pollen grains through meiosis and mitosis. RNA-seq and proteome association analysis indicated that fatty acids were converted to sucrose after the 6-mm spikelet stage, based on the abundance of most key enzymes of the glyoxylate cycle and gluconeogenesis. In conclusion, our study provides novel insights into the pollen viability of O. longistaminata at the transcriptome level, which can be used to improve the efficiency of male parent pollination in hybrid rice breeding applications.

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