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Seriola quinqueradiata

ABSTRACT: Yellowtail genome analysis

PROVIDER: PRJDB5349 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	BDJM01.dat.gz	Other
	BDJM01.fasta.gz	Fasta.gz
	BDJM01.master.dat	Other
	BDJM01.dat.gz	Other

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1 - 4 of 4

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Seriola quinqueradiata

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Seriola quinqueradiata

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Seriola quinqueradiata

Project description:The haploid-based draft genome assembly and annotation of the yellowtail (Seriola quinqueradiata)

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Project description:Sequencing for QTL region associated with resistance to Benedenia disease in linkage group Squ2 of the Japanese yellowtail/amberjack, Seriola quinqueradiata

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Seriola quinqueradiata

Project description:Seriola quinqueradiata overview

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The yellowtail (Seriola quinqueradiata) genome and transcriptome atlas of the digestive tract.

Project description:Seriola quinqueradiata (yellowtail) is the most widely farmed and economically important fish in aquaculture in Japan. In this study, we used the genome of haploid yellowtail fish larvae for de novo assembly of whole-genome sequences, and built a high-quality draft genome for the yellowtail. The total length of the assembled sequences was 627.3 Mb, consisting of 1,394 scaffold sequences (>2 kb) with an N50 length of 1.43 Mb. A total of 27,693 protein-coding genes were predicted for the draft genome, and among these, 25,832 predicted genes (93.3%) were functionally annotated. Given our lack of knowledge of the yellowtail digestive system, and using the annotated draft genome as a reference, we conducted an RNA-Seq analysis of its three digestive organs (stomach, intestine and rectum). The RNA-Seq results highlighted the importance of certain genes in encoding proteolytic enzymes necessary for digestion and absorption in the yellowtail gastrointestinal tract, and this finding will accelerate development of formulated feeds for this species. Since this study offers comprehensive annotation of predicted protein-coding genes, it has potential broad application to our understanding of yellowtail biology and aquaculture.

| S-EPMC6191305 | biostudies-literature

Taste Evaluation of Yellowtail (Seriola Quinqueradiata) Ordinary and Dark Muscle by Metabolic Profiling.

Project description:We performed metabolic profiling on yellowtail (Seriola quinqueradiata) muscle to develop an objective taste evaluation method for fish meat. Dark (DM) and ordinary (OM) muscle samples before and after storage were subjected to gas chromatography-mass spectrometry (GC-MS) analysis and taste measurements using an electronic tongue. The metabolites identified by the GC-MS analysis were treated as x variables, and the taste values obtained by the electronic tongue were treated as y variables. The relationships between the metabolites and taste attributes were evaluated by two-way orthogonal projections to latent structures (O2PLS) analysis. The O2PLS analyses were normalized in two ways, unit variance (UV) and pareto (Par) scaling. The O2PLS (UV) analysis produced 3+1+0 models in Autofit and this model was statistically significant with R2Y (0.73) and Q2 (0.52) metrics. In particular, significant correlations were found between DM or OM and metabolite intensity and taste attributes, and strong associations were found between "sourness" and lysine, "irritant" and alanine and phenylalanine, "saltiness" and pantothenic acid, and "umami" and creatinine and histidine. The O2PLS (Par) analysis of DM generated significant predictive models for "acidic bitterness," "irritant," "saltiness," "bitterness," "astringency," and "richness." Among these, only "irritant" was affected by storage. This method was thus effective in evaluating the taste of yellowtail muscle.

| S-EPMC6680873 | biostudies-literature

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