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Oreochromis niloticus

ABSTRACT: Differential gene expression profiles and alternative splicing events in the gill tissues of the Nile tilapia in response to acute hypoxia

PROVIDER: PRJDB5451 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR083608_1.fastq.gz	Fastqsanger.gz
	DRR083608_2.fastq.gz	Fastqsanger.gz
	DRR083609_1.fastq.gz	Fastqsanger.gz
	DRR083609_2.fastq.gz	Fastqsanger.gz
	DRR083610_1.fastq.gz	Fastqsanger.gz

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Oreochromis niloticus

Project description:Transcriptomic analysis reveals abundant differential gene expression and alternative splicing events in the heart tissues of the Nile tilapia in response to acute hypoxia

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Oreochromis niloticus

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Oreochromis niloticus

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Oreochromis niloticus

Project description:Nile Tilapia Oreochromis niloticus sample Raw sequence reads

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Transcriptome analysis of miRNA–mRNA interactions revealed regulation of multiple signaling pathways in genetically improved farmed tilapia (GIFT, Oreochromis niloticus) gill tissues under hypoxia stress

Project description:Dissolved oxygen (DO) is the basis of fish survival, and proper DO level is an important condition to ensure the normal growth of fish. Hypoxic environment is prone to disturb the normal breathing and metabolism of fish, which in turn affects their growth and survival. Gill tissue is the respiratory organs of fish and is in direct contact with the external environment. However, there are few reports on the molecular regulatory mechanism of genetically improved farmed tilapia (GIFT, Oreochromis niloticus) gill tissues in response to hypoxia. Here, we first examine the hypoxia-induced damage of gill tissue by hematoxylin-eosin staining, and then constructed miRNA and mRNA libraries of GIFT gill tissue at 96h of hypoxia stress by a high-throughput sequencing technology, each library has three biological replicates. Gill lamellae of GIFT showed capillary rupture and red blood cell enlargement and overflow under hypoxia stress. Transcription sequencing results showed that the clean reads of miRNA libraries were 9,627,953-13,544,660; the clean reads of mRNA libraries were 43,817,776-53,130,102. Based on the miRNA-mRNA pairs screening principles and mRNA sequencing results, we selected and verified seven differentially expressed miRNAs and their potential target genes. The sequencing results were consistent with the qRT-PCR validation results. These selected miRNA-mRNA pairs are mainly concentrated in the signaling pathways of immune response and metabolic regulation. This study provides new insights into the mechanisms of fish adaptation under hypoxic stress.

2023-02-22 | GSE145788 | GEO

2023-02-26 | GSE146050 | GEO

Oreochromis niloticus

Project description:Transcriptome sequencing of Nile tilapia hepatopancreas in different salinity

| PRJNA280611 | ENA

Oreochromis niloticus niloticus

Project description:Global epigenetic analysis of Nile tilapia under high temperature induced masculinization

| PRJNA290224 | ENA

Nile tilapia (Oreochromis niloticus) gene expression profiling in males and females at early embryonic development (2, 5 and 9 days post fertilization )

Project description:Commercial production of tilapia relies on monosex cultures of males, which so far proved difficult to maintain in large scale production facilities. Thus, a better understanding of the genetic architecture of the complex trait of sex determination in tilapia is needed.We aimed to detect genes that were differentially expressed by gender at early embryonic development. Artificial fertilization of O. niloticus females with either sex-reversed males (ΔXX) or genetically-modified YY 'supermales' resulted in all-female and all-male embryos, respectively. Pools of all-female and all-male embryos at 2, 5 and 9 days post fertilization were used for custom Agilent eArray. 56 pool samples of Nile tilapia full siblings groups (female or male) at day 2, 5 or 9 post fertilization were subjected to total RNA extraction from whole embryo tissues and hybridized to the custom Agilent array. Each sample was yielded from different cross of artificial fertilization: six dams X five sires. The resulting gender were known based on the sire, sex-reversed males (ΔXX) or genetically-modified YY 'supermales' resulted in all-female and all-male embryos, respectively.

2014-11-01 | E-GEOD-50974 | biostudies-arrayexpress

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