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Homo sapiens strain:HEK293T/17 cells

ABSTRACT: Identification of transcription start site of SINEUP-GFP

PROVIDER: PRJDB5492 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR086873.fastq.gz	Fastqsanger.gz
	DRR086874.fastq.gz	Fastqsanger.gz
	DRR086875.fastq.gz	Fastqsanger.gz
	DRR086876.fastq.gz	Fastqsanger.gz
	DRR086877.fastq.gz	Fastqsanger.gz

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Detection of SINEUP RNA modification by Oxford Nanopore direct-RNA sequencing

Project description:To detect the modifed bases in SINEUP RNA, we compared chemically modified in vitro transcribed (IVT) SINEUP-GFP RNA and in-cell transcribed (ICT) SINEUP RNA from SINEUP-GFP and sense EGFP co-transfected HEK293T/17 cells. Comparative study of Nanopore direct RNA sequencing data from non-modified and modified IVT samples against the data from ICT SINEUP RNA sample revealed modified k-mers positions in SINEUP RNA in the cell.

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