Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Escherichia coli strain:strain K-12 substrain DH10B

ABSTRACT: Single molecule sequencing after WGA

PROVIDER: PRJDB5917 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR099847_consensus.fastq.gz	Fastqsanger.gz
	DRR099848_consensus.fastq.gz	Fastqsanger.gz
	DRR099849_consensus.fastq.gz	Fastqsanger.gz
	DRR099850_consensus.fastq.gz	Fastqsanger.gz
	DRR099851_consensus.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 35

Similar Datasets

Escherichia coli strain:K-12 DH10B

Project description:Escherichia coli strain:K-12 DH10B Raw sequence reads

| PRJNA752926 | ENA

Escherichia coli

Project description:Mapping DNA polymerase errors by Single-molecule Sequencing

| PRJNA310757 | ENA

Escherichia coli str. K-12 substr. DH10B

Project description:E Coli, substrain DH10B and U2OS Genome sequencing

| PRJNA191052 | ENA

Escherichia coli str. K-12 substr. DH10B

Project description:Escherichia coli str. K-12 substr. DH10B Genome sequencing

| PRJNA217292 | ENA

Transcriptional profile of Escherichia coli K12 strain DH10B harboring A1501 nitrogen fixation island (NFI) under nitrogen fixation conditions

Project description:A1501 NFI is a genomic island derived from Pseudomonas stutzeri A1501. To study the molecular interactions of the P. stutzeri nif genes with the E. coli genome during nitrogen fixation, the NIF of A1501 was transferred into E. coli and comparative transcriptomics analyses were performed between nitrogen fixation conditions and nitrogen excess conditions.

2015-08-05 | GSE37780 | GEO

Closed Genome Sequence of Escherichia coli K-12 Group Strain C600.

Project description:Escherichia coli strain C600 is a prototypical K-12 derived laboratory strain which has been broadly used for molecular microbiology and bacterial physiology studies since its isolation in 1954. Here, we present the closed genome sequence of E. coli strain C600, retrieved from the American Type Culture Collection (ATCC 23724).

| S-EPMC6328645 | biostudies-literature

The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse.

Project description:Escherichia coli DH10B was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids. The strain was constructed by serial genetic recombination steps, but the underlying sequence changes remained unverified. We report the complete genomic sequence of DH10B by using reads accumulated from the bovine sequencing project at Baylor College of Medicine and assembled with DNAStar's SeqMan genome assembler. The DH10B genome is largely colinear with that of the wild-type K-12 strain MG1655, although it is substantially more complex than previously appreciated, allowing DH10B biology to be further explored. The 226 mutated genes in DH10B relative to MG1655 are mostly attributable to the extensive genetic manipulations the strain has undergone. However, we demonstrate that DH10B has a 13.5-fold higher mutation rate than MG1655, resulting from a dramatic increase in insertion sequence (IS) transposition, especially IS150. IS elements appear to have remodeled genome architecture, providing homologous recombination sites for a 113,260-bp tandem duplication and an inversion. DH10B requires leucine for growth on minimal medium due to the deletion of leuLABCD and harbors both the relA1 and spoT1 alleles causing both sensitivity to nutritional downshifts and slightly lower growth rates relative to the wild type. Finally, while the sequence confirms most of the reported alleles, the sequence of deoR is wild type, necessitating reexamination of the assumed basis for the high transformability of DH10B.

| S-EPMC2293198 | biostudies-literature

Finished Genome Sequence of Escherichia coli K-12 Strain HMS174 (ATCC 47011).

Project description:Escherichia coli strain K-12 substrain HMS174 is an engineered descendant of the E. coli K-12 wild-type strain. Like its ancestor, it is an important organism in biotechnological research and is used in fermentation processes for heterologous protein production. Here, we report the complete genome sequence of E. coli HMS174 (ATCC 47011).

| S-EPMC4239344 | biostudies-literature

Escherichia coli str. K-12 substr. MG1655

Project description:Digital RNA Sequencing Minimizes Sequence-Dependent Bias and Amplification Noise with Optimized Single Molecule Barcodes

| PRJNA151211 | ENA

Escherichia coli strain 8624 and Escherichia coli strain VS94 with signaling molecules

Project description:These E. coli strains were grown with various signaling molecules and the expression profiles were determined. Keywords: addition of quorum and host hormone signals

2007-09-20 | GSE7439 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data