Project description:The enslavement of foreign organelles by protists for metabolic gain is a common phenomenon within aquatic ecosystems. Ciliates belonging to the Mesodinium rubrum species complex are unique in that they also steal a transcriptionally active prey nucleus, the kleptokaryon, from certain cryptophytes, enabling control and replication of stolen plastids and other organelles. Here we show that kleptokaryon-containing M. rubrum undergo the process of photoacclimation, changing pigment concentrations in response to light in a manner similar to their cryptophyte prey, Geminigera cryophila. The proteome and transcriptome of the G. cryophila nucleus were analyzed in M. rubrum (i.e. kleptokaryon) and in the free-living cryptophyte under changing light conditions.
Project description:In order to investigate the physiological and biochemical characteristics and molecular mechanisms during the leaf colour change of Acer rubrum L, this study used Acer rubrum L. 'Autumn Blaze' cuttings as material and analysed the transcriptome and miRNAs of Acer rubrum L leaves under different light and temperature treatments. The transcriptome and miRNAs of Acer rubrum L leaves were analysed under different light and temperature treatments, and miRNA-mRNA association analysis was performed for the differentially expressed mRNAs and miRNAs.