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Hordeum vulgare

ABSTRACT: RNA-seq analysis in callus of barley

PROVIDER: PRJDB7393 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR149321.fastq.gz	Fastqsanger.gz
	DRR149322.fastq.gz	Fastqsanger.gz
	DRR149323.fastq.gz	Fastqsanger.gz
	DRR149324.fastq.gz	Fastqsanger.gz
	DRR149325.fastq.gz	Fastqsanger.gz

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Hordeum vulgare Transcriptome or Gene expression

Project description:We hypothesized that the genome segments of cultivated barley should show certain similarity with its ancestral wild barley. Instead of whole genome sequences, we employed RNA-Seq to investigated the genomic origin of modern cultivated barley using some representative wild barley genotypes from the Near East and Tibet, and representative world-wide selections of cultivated barley.

2014-10-06 | E-MTAB-4221 | biostudies-arrayexpress

Hordeum vulgare Transcriptome or Gene expression

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Hordeum vulgare strain:landrace Transcriptome or Gene expression

Project description:In the present study, we investigated the transcriptome features during hulless barley grain development. Using Illumina paired-end RNA-Sequencing, we generated two data sets of the developing grain transcriptomes from two hulless barley landraces.

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Genome-Wide Analysis of the MADS-box Gene Family Involved in Salt and Waterlogging Tolerance in Barley (Hordeum Vulgare. L)

Project description:To investigate the salt and waterlogging mechanism of barley, we chose salt and waterlogging sensitive and tolerant varieties as subjects. We then performed gene expression profiling analysis using data obtained from RNA-seq of two varieties at two three points.

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Hordeum vulgare

Project description:Hulless barley DAP-seq rawdata

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RNA-Seq data analysis of Hordeum vulgare cv. Morex transcriptome to study photomorphogenesis

Project description:Hordeum vulgare is one of the first domesticated grains in the world and it has been reported that variations in the light environment have a substantial effect on barley plant development and biological processes. High-throughput RNA-Seq study was performed to investigate the complex transcriptome network required for photomorphogenesis in barley. Seedlings were grown in dark and light conditions and three biological replicates were sampled from each condition. Six libraries from poly-A rich mRNA fraction were subjected to 51bp single-end RNA-seq sequencing.

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The primary transcriptome of barley (Hordeum vulgare L.) chloroplasts

Project description:Gene expression in plastids of higher plants is dependent on two different transcription machineries, a plastid-encoded bacterial-type RNA polymerase (PEP) and a nuclear-encoded phage-type RNA polymerase (NEP), which recognize distinct types of promoters. The division of labor between PEP and NEP during plastid development and in mature chloroplasts is unclear due to a lack of comprehensive information on promoter usage. Here we present a thorough investigation into the distribution of PEP and NEP promoters within the plastid genome of barley (Hordeum vulgare L). Using a novel differential RNA sequencing approach, which discriminates between primary and processed transcripts, we obtained a genome-wide map of transcription start sites in plastids of mature first leaves. PEP-lacking plastids of the albostrians mutant allowed for the unambiguous identifications of NEP promoters. We observed that the chloroplast genome contains many more promoters than genes. According to our data, most genes (including genes coding for photosynthesis proteins) have both PEP and NEP promoters. We also detected numerous transcription start sites within operons indicating transcriptional uncoupling of genes in polycistronic gene clusters. Moreover, we mapped many transcription start sites in intergenic regions, as well as opposite to annotated genes demonstrating the existence of numerous non-coding RNA candidates. dRNA-seq analysis of total RNA from green and white plastids of the barley mutant line albostrians

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