Project description:We identified genome-wide binding regions of NdgR in Streptomyces coelicolor using chromatin immunoprecipitation sequencing (ChIP-seq). We constructed 6×myc-tagged NdgR strain using homologous recombination with myc-tagging vector. Analysis of the sequencing data aligned to Streptomyces coelicolor genome database (NC_003888).
Project description:This study compared the genome of Streptomyces rimosus rimosus against that of Streptomyces coelicolor. It also compared 4 strains with changes in oxytetracycline production and derived from G7, the type strain, against G7. Keywords: Comparative genomic hybridization
Project description:Investigation of whole genome gene expression level changes in Streptomyces avermitilis delta-aveI mutant, compared to the wild-type strain. The mutants analyzed in this study are further described in Chen L, Lu Y., Chen J, Zhang W, Shu D, Qin Z, Yang S, Jiang W. (2008) Characterization of a negative regulator AveI for avermectin biosynthesis in Streptomyces avermitilis NRRL8165. Appl Microbiol Biotechnol 80(2): 277-86.
Project description:Streptomyces bingchenggensis is a soil bacterium that produces milbemycins. Milbemycins and their derivatives are valuable biopesticides in the agricultural field. Owing to their advantages such as high efficiency and safety for human and animal,it was urgent to construct high-yield strain to ensure low production cost. To obtain genes closely correlated with milbemycin production, we have compared the whole genome microarray expression profiling of two strains (the parent one strain and high-yielding strain). In Streptomyces bingchenggensis, there are abundant exporters, which are responsible for transporting various substrates. In the result, some drug exporters were chosen to enhance production of milbemycin .
Project description:Streptomyces bingchenggensis is a soil bacterium that produces milbemycins. Milbemycins are commercially insecticidal and acaricidal antibiotics in agriculture, owing to their advantages such as high efficiency and safety for human and animal. To obtain genes valuable for further improvement of titer, we have compared two strains（the parental strain and high-yielding strain）by whole genome microarray expression profiling as a discovery platform. In Streptomyces bingchenggensis, there are abundant transporters, which are responsible for transporting various substrates. In the result, some sugar transporter genes showed significant gene expression.
Project description:This project aims to discover novel bioactive compounds from Streptomyces isolated from the rhizosphere from wild medicinal plants from Hamedan province, Iran. Proteomics is used to assist in discovery and characterization of the compounds. Streptomyces isolates are grown on ISP-4 medium for three days, proteins were extracted and analysed by shotgun proteomics.
Project description:FK506 (tacrolimus) is a valuable immunosuppressant produced by several Streptomyces strains. In the genome of the wild type producer Streptomyces tsukubaensis NRRL18488 FK506 biosynthesis is encoded by a gene cluster that spans 83.5 kilobases. A whole transcriptome differential shotgun sequencing of S. tsukubaensis was performed to analyze transcription at two different time points; before and during active FK506 production. In total 8,914 transcription start sites were identified in either condition, which enabled precise determination of the 5'-UTR length of the corresponding transcripts as well as the identification of two consensus sequence motifs in the promoter regions. The transcription start sites of all gene operons within the FK506 cluster were identified, including three examples of leaderless RNA transcripts. These data provide detailed insight into the transcription of the FK506 biosynthetic gene cluster and supports future regulatory studies and genetic manipulations.
