Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature.
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature.
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature.
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature. Ninety-eight samples from three separate temperature exposure studies were analyzed on ninety-eight microarrays, using a common reference design, with multiple biological replicates for each temperature condition for each year of the experiment.
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature. Ninety-eight samples from three separate temperature exposure studies were analyzed on ninety-eight microarrays, using a common reference design, with multiple biological replicates for each temperature condition for each year of the experiment.
Project description:Barley cv. Morex inoculated with Fusarium graminearum (isolate Butte 86) or water (mock). Sampled at 24, 48, 72, 96 and 144 hours after treatment. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Jayanand Boddu. The equivalent experiment is BB9 at PLEXdb.]
2011-11-22 | GSE33407 | GEO
Project description:Rosa hybrida Raw sequence reads
Project description:Water-deficit stress negatively affects wheat yield and quality. Abiotic stress on the parental plants during reproduction could have transgenerational effects on the progenies. Here we investigated the transgenerational influence of pre-anthesis water-deficit stress by detailed analysis of the yield components, grain quality traits, and physiological traits in durum wheat. Next-generation sequencing analysis profiled the small RNA-omics, mRNA transcriptomics, and mRNA degradomics in the progenies. Parental water-deficit stress had positive impacts on the progenies in certain traits like harvest index and protein content in given genotype. Small RNA-seq identified 1739 conserved and 774 novel microRNAs (miRNAs). Transcriptome-seq characterised the expression of 66,559 genes while degradome-seq profiled the miRNA-guided mRNA cleavage dynamics. Differentially expressed miRNAs and genes were identified, with significant regulatory patterns subject to trans- and inter- generational stress. Integrated analysis based on the three omics revealed the significant biological interactions between stress-responsive miRNA and targets, with possible contributions towards transgenerational stress tolerance via pathways such as hormone signalling and nutrient metabolism. Our study provides the first confirmation of the transgenerational effects of water-deficit stress in durum wheat. New insights gained on the molecular level indicate that key miRNA-mRNA modules are potential candidates in transgenerational stress improvement.