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Cricetulus griseus strain:CHO K1

ABSTRACT: Thermal hysteresis in CHO K1, a Chinese Hamster (Cricetulus griseus) Ovary derived established cell line

PROVIDER: PRJDB7873 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR164940.fastq.gz	Fastqsanger.gz
	DRR164941.fastq.gz	Fastqsanger.gz
	DRR164942.fastq.gz	Fastqsanger.gz
	DRR164943.fastq.gz	Fastqsanger.gz
	DRR164944.fastq.gz	Fastqsanger.gz

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Project description:In biopharmaceutical production, Chinese hamster ovary (CHO) cells derived from Cricetulus griseus remain the most commonly used host cell for recombinant protein production, especially antibodies. Over the last decade in-depth multi-omics characterization of these CHO cells provided data for extensive cell line engineering and corresponding increases in productivity. exosomes, extracellular vesicles containing proteins and nucleic acids, are barely researched at all in CHO cells. Exosomes have been proven to be an ubiquitous mediator of intercellular communication and are proposed as new biopharmaceutical format for drug delivery, indicator reflecting host cell condition and anti-apoptotic factor in spent media. Here we sequenced non-coding RNA of Exosomes (EXO) and whole cell lysate (WCL) isolated from CHO-K1 Cell Cultures at different growth phases (logarithmic/exponential phase (log/exp), stationary phase (stat), as well as death phase at 80 % viability (80 % ) and 60 % viability (60 %)) via Lexogen Small RNA-Seq Library Prep Kit for Illumina on the Illumina MiSeq platform in PE mode 2 x 36nt.

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