Project description:To identify the most important gene(s) for maintaining the stem cell properties of leukemia stem cells (LSCs), we analyzed the transcriptional profiles of LSC and non-LSC subsets from acute leukemia patients and identified S1PR1 as a novel biomarker of LSCs. Then to understand the underlying mechanisms of S1PR1-dependent stemness maintenance, we performed gene expression profiling analyses comparing CD34+S1PR1+ and CD34+S1PR1- cell fractions from AML patients and healthy donors; we also determined the global transcriptional response of KG1 cells to an inhibitor of S1PR1 (FTY720) at two concentrations.
Project description:RNA-Sequencing of 27 functionally validated LSC and blast fractions from 9 AML patients. Three healthy hematopoietic stem and progenitor cells from age-matched controls.
Project description:Global gene expression comparison between mesenchymal stem cells (MSCs) purified from the BM of AML patients versus healthy donors.
