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Homo sapiens

ABSTRACT: Direct conversion from human dermal fibroblasts into urothelial cells by defined factors

PROVIDER: PRJDB8092 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR174811_1.fastq.gz	Fastqsanger.gz
	DRR174811_2.fastq.gz	Fastqsanger.gz
	DRR174812_1.fastq.gz	Fastqsanger.gz
	DRR174812_2.fastq.gz	Fastqsanger.gz
	DRR174813_1.fastq.gz	Fastqsanger.gz

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Project description:In search for factors, overexpression of which in human dermal fibroblasts causes direct conversion to cells similar to keratinocytes, micro RNA expression profiles of human primary keratinocytes and human primary dermal fibroblasts are investigated. Skin samples obtained from 3 different sites of 1 subject were used for establishment of 3 primary keratinocytes and 3 primary dermal fibroblasts. Thus obtained 3 primary keratinocytes and primary dermal fibroblasts underwent micro RNA profiling.

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Direct Cell Conversion of Human Fibroblasts to Monocytic phagocytes by Forced Expression of Monocytic Regulatory Network Elements

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Project description:We report the generation of myelinating human oligodendrocytes from dermal fibroblasts using a direct lineage conversion technique.

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The global gene expression pattern between human fibroblasts and human neural stem cells

Project description:Recent advances in direct reprogramming using cell type-specific transcription factors provide an unprecedented opportunity for rapid generation of desired human cell types from easily accessible tissues. However, due to the diversity of conversion factors that facilitate the process, an arduous screening step is inevitable to find the appropriate combination(s). Here, we show that under chemically defined conditions minimal pluripotency factors are sufficient to directly reprogram human fibroblasts into stably self-renewing neural progenitor/stem cells (NSCs), but without passing through a pluripotent intermediate stage. These NSCs can be expanded and propagated in vitro without losing their potential to differentiate into various neuronal subtypes and glia. Our direct reprogramming strategy represents a simple and advanced paradigm of direct conversion that will provide an unlimited source of human neural cells for cell therapy, disease modeling, and drug screening. We used microarray to compare the global gene expression pattern between human fibroblasts and human neural epitheliums from human ESCs or directly from fibroblasts. We cultured cells and harvested them and then extracted total RNA for microarray.

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