Project description:Tea (Camellia sinensis (L.) O. Kuntze) is an important non-alcoholic commercial beverage crop. Tea tree is a perennial plant, and winter dormancy is its part of biological adaptation to environmental changes. We recently discovered a novel tea tree cultivar that can generate tender shoots in winter, but the regulatory mechanism of this ever-growing tender shoot development in winter is not clear. In this study, we conducted a proteomic analysis for identification of key genes and proteins differentially expressed between the winter and spring tender shoots, to explore the putative regulatory mechanisms and physiological basis of its ever-growing character during winter.
Project description:The transcriptome of a light sensitive tea cultivar ‘Huangjinya’ plants exposed to sunlight and shade were analyzed by high-throughput sequencing followed by de novo assembly.
Project description:Tea plants (Camellia sinensis) present an excellent system to study evolution and diversification of specialized metabolites due to their abundance in classes, numbers and contents. A large number of tea cultivars have been cultivated throughout the world not only because of their adaption to different environments but of selection for specific flavors. The chemical and genetic basis for unique taste and aroma of different tea cultivars remains largely unknown, but is critical for guiding genetic breeding of new cultivars. Using transcriptomic data from 136 representative tea accessions in China, we obtain 925,854 high-quality single-nucleotide polymorphisms (SNPs) useful for marker-assisted breeding. Phylogenetic and population structure analyses separate sampled tea accessions into five major groups. Different major alleles are identified on 1183 SNP sites for the two major types of tea, C. sinensis var. assamica (CSA) and C. sinensis var. sinensis (CSS), reflecting fixation of these alleles after population divergence. Non-targeted metabolomic analyses detect 2,818 and 2,311 metabolic features in tea samples in positive and negative ionization modes, respectively, including 355 and 286 metabolites respectively that are differentially accumulated in different tea groups. Each phylogenetic group contains signature metabolites. In particular, CSA tea accessions are featured with high accumulation of diverse classes of flavonoid compounds, such as flavanols, flavonol mono-/di-glycosides and proanthocyanidin dimers. Comparisons of gene expression profiles of different tea groups identify hundreds of differentially expressed genes with some involved in the biosynthesis of characteristic tea metabolites, reflecting a combinational effect of genetic and environmental factors. Taken together, our study provides new insights into the phylogenetic relationships, molecular markers, metabolite compositions, and gene expression profiles of representative cultivated tea accessions in China, which are beneficial for targeted tea breeding and improvement.
Project description:Background: Lysine crotonylation (Kcr), as a novel evolutionarily conserved type of PTM, is ubiquitous and essential in cell biology. However, its functions in tea plant, an important beverage crop, are largely unknown. Our study firstly attempted to describe Kcr proteins in tea leaves under NH4+ deficiency/resupply, and provided significant insights into exploring the physiological role of Kcr in plants for N utilization. Results: We performed the global analysis of crotonylome in tea plants under NH4+ deficiency/resupply using high-resolution LC-MS/MS coupled with highly sensitive immune-antibody. A total of 2288 kcr sites on 971 proteins were identified, of which contained in 15 types of Kcr motifs. Most of Kcr proteins were located in chloroplast and cytoplasm. 120 and 151 Kcr proteins were significantly changed at 3 hours and 3days of NH4+ resupply, respectively. Bioinformatics analysis showed that differentially expressed Kcr proteins participated in diverse biological processes such as photosynthesis, carbon fixation and amino acid metabolism, suggesting Kcr plays important roles in these processes. Interestingly, a large number of enzymes were crotonylated, and the activity and Kcr level of these enzymes changed significantly after NH4+ resupply, indicating a potential function of Kcr in the regulation of enzyme activities. Moreover, the protein-protein interaction analysis revealed that the diverse interactions of identified Kcr proteins mainly involved in photosynthesis, carbon fixation, amino acid metabolism and ribosome. Conclusions: The results suggested that lysine crotonylated proteins might play regulating roles in metabolic process in tea leaves under NH4+ deficiency/resupply. The critical regulatory roles mainly involved in diverse aspects of primary metabolic processes, especially in photosynthesis, carbon fixation and amino acid metabolism. The provided data may serve as important resources for exploring the physiological, biochemical, and genetic role of lysine crotonylation in tea plants.
Project description:The RNA-Seq was used to analyze the expression profiling of genes in different ablescent stages of 'Anji Baicha' Examination of three tea leaf samples in yellow stage, white stage and green stage
Project description:In this study, it is noticeable that 32 tea-specific miRNAs were confirmed on the base of genome survey, using deep sequencing and microarray hybridization, and many miRNAs might associate with secondary metabolites synthesis. Leaves, buds and roots were collected
Project description:In this study, it is noticeable that 32 tea-specific miRNAs were confirmed on the base of genome survey, using deep sequencing and microarray hybridization, and many miRNAs might associate with secondary metabolites synthesis.
