Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Aspergillus oryzae RIB40

ABSTRACT: Time-course mRNA-Seq analysis of Aspergillus oryzae RIB40 and the faaA disruptant grown in the presence of 1% Triton X-100

PROVIDER: PRJDB8293 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR178650_1.fastq.gz	Fastqsanger.gz
	DRR178650_2.fastq.gz	Fastqsanger.gz
	DRR178651_1.fastq.gz	Fastqsanger.gz
	DRR178651_2.fastq.gz	Fastqsanger.gz
	DRR178652_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 72

Similar Datasets

Aspergillus oryzae RIB40

Project description:Expression data from Aspergillus oryzae grown under hypoxic condition.

| PRJNA144865 | ENA

Aspergillus oryzae RIB40

Project description:Expression analysis of Aspergillus oryzae grown on different carbon sources

| PRJNA212019 | ENA

Effects of manR disruption and overexpression on Aspergillus oryzae gene expression

Project description:Fungal endo-M-NM-2-mannanases (M-NM-2-mannanases) are widely used as industrial enzymes; however, no transcriptional regulator of M-NM-2-mannanases has been identified in fungi or other eukaryotic cells to date. To identify a transcriptional regulator of M-NM-2-mannanases in Aspergillus oryzae, a gene-disruptant library of transcriptional regulators was screened for mutants exhibiting reduced M-NM-2-mannanase activity by using konjac glucomannan as the substrate, and ManR, a Zn(II)2Cys6 type DNA binding protein was identified. Moreover, a manR-overexpressing strain showed significantly increased M-NM-2-mannanase activity. DNA microarray analysis of the manR-disruptant strain and the manR-overexpressing strain further indicated that when konjac glucomannan is used as the carbon source, ManR positively regulates the gene expression of not only M-NM-2-mannanase, but also the enzymes involved in the degradation of galactomannans and glucomannans such as M-NM-1-galactosidase, M-NM-2-mannosidase, acetylmannan esterase, and M-NM-2-glucosidase. Therefore, we conclude that ManR is a positive regulator of the M-NM-2-mannan utilization system in A. oryzae. manR disruptant, manR-overexpressing strain and A. oryzae RkuptrP2-1M-bM-^HM-^FAF/P (derivative of A. oryzae RIB40) were cultivated in minimal medium containing 1% konjac glucomannan as the sole carbon source. After 6 h cultivation, total RNAs from the mycelia were extracted, and DNA microarray analysis was carried out. The analysis of manR disruptant was conducted with 4 biological replications, the analysis of manR overexpressing strain was conducted with 3 biological replications.

2012-11-08 | E-GEOD-40590 | biostudies-arrayexpress

GNPS Aspergillus oryzae RIB40 in different media

Project description:Aspergillus oryzae RIB40 grown in YPD, AF6 and EG:JM media

2022-07-18 | MSV000089904 | GNPS

Effects of manR disruption and overexpression on Aspergillus oryzae gene expression

Project description:Fungal endo-β-mannanases (β-mannanases) are widely used as industrial enzymes; however, no transcriptional regulator of β-mannanases has been identified in fungi or other eukaryotic cells to date. To identify a transcriptional regulator of β-mannanases in Aspergillus oryzae, a gene-disruptant library of transcriptional regulators was screened for mutants exhibiting reduced β-mannanase activity by using konjac glucomannan as the substrate, and ManR, a Zn(II)2Cys6 type DNA binding protein was identified. Moreover, a manR-overexpressing strain showed significantly increased β-mannanase activity. DNA microarray analysis of the manR-disruptant strain and the manR-overexpressing strain further indicated that when konjac glucomannan is used as the carbon source, ManR positively regulates the gene expression of not only β-mannanase, but also the enzymes involved in the degradation of galactomannans and glucomannans such as α-galactosidase, β-mannosidase, acetylmannan esterase, and β-glucosidase. Therefore, we conclude that ManR is a positive regulator of the β-mannan utilization system in A. oryzae.

2012-11-08 | GSE40590 | GEO

Aspergillus oryzae strain:RIB40

Project description:Aspergillus oryzae RIB40

| PRJDB17490 | ENA

Aspergillus oryzae RIB40

Project description:Aspergillus oryzae RIB40 Genome sequencing and assembly

| PRJNA277168 | ENA

Aspergillus oryzae RIB40

Project description:Mycelial penetration in rice koji and nuclear increase of Aspergillus oryzae

| PRJDB9259 | ENA

Aspergillus oryzae RIB40

Project description:Effects of manR disruption and overexpression on Aspergillus oryzae gene expression