Project description:Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine. It has been shown to relieve the heat stress of beef cattle by improving antioxidant activity and rumen microbial protein synthesis, but the mechanism of CrPyr influencing rumen fermentation remains unclear. This study aimed to use metaproteomics technologies to investigate the bacterial protein function in rumen fluid samples taken from heat-stressed beef cattle treated with or without 60 g/d CrPyr.