Project description:Macrophages were derived from the bone-marrow of 3 x fl/+ Dicer LysCre +/- (wild-type) and 3 x fl/fl Dicer LysCre +/- mice and stimulated with IL-4 (50ng/mL) for 72h. Total RNA was isolated and analyzed by gene array. In this experiment, we derived Dicer deficient bone-marrow macrophages using Dicer fl/+ LysM-Cre by Dicer fl/+ crossed mice to obtain Dicer fl/fl LysM-cre progeny (and Dicer deficient macrophages). Next, we studied the effects of IL-4 stimulation in macrophage with a deficiency in Dicer/microRNAs.
Project description:Quantitative gene expression analysis of early postnatal hippocampal development in wild-type and Pol?-deficient mice using RNA-seq
Project description:In an attempt to clarify the diferrence of exosomal microRNA derived from Normal BM and primary AML CD34+ cells. Primary cells were cultured in serum free medium and supernatant was harvested. After extract microRNAs, the difference of exosomal microRNAs between normal BM and leukemia was analyzed by array.