Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mus musculus strain:C57BL/6TS2

ABSTRACT: Gene expression profiling of mouse trophoblast stem cells treated with small molecular compounds

PROVIDER: PRJDB9519 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR217281.fastq.gz	Fastqsanger.gz
	DRR217282.fastq.gz	Fastqsanger.gz
	DRR217283.fastq.gz	Fastqsanger.gz
	DRR217284.fastq.gz	Fastqsanger.gz
	DRR217285.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

Mus musculus

Project description:Pluripotent Stem Cells Induced from Mouse Neural Stem Cells and Small Intestinal Epithelial Cells by Small Molecule Compounds

| PRJNA304463 | ENA

Establishment of trophoblast stem cells under defined culture conditions in mice

Project description:The inner cell mass (ICM) and trophoblast cell lineages duet early embryonic development in mammals. After implantation, the ICM forms the embryo proper as well as some extraembryonic tissues, whereas the trophoectoderm (TE) exclusively forms the fetal portion of the placenta and the trophoblast giant cells. Although embryonic stem (ES) cells can be derived from ICM in cultures of mouse blastocysts in the presence of LIF and/or combinations of small-molecule chemical compounds, and the undifferentiated pluripotent state can be stably maintained without use of serum and feeder cells, defined culture conditions for derivation and maintenance of undifferentiated trophoblast stem (TS) cells have not been established. Here, we report that addition of FGF2, activin A, XAV939, and Y27632 are necessary and sufficient for derivation of TS cells from both of E3.5 blastocysts and E6.5 early postimplantation extraembryonic ectoderm. Moreover, the undifferentiated TS cell state can be stably maintained in chemically defined culture conditions. Cells derived in this manner expressed TS cell marker genes, including Eomes, Elf5, Cdx2, Klf5, Cdh1, Esrrb, Sox2, and Tcfap2c; differentiated into all trophoblast subtypes (trophoblast giant cells, spongiotrophoblast, and labyrinthine trophoblast) in vitro; and exclusively contributed to trophoblast lineages in chimeric animals. This delineation of minimal requirements for derivation and self-renewal provides a defined platform for precise description and dissection of the molecular state of TS cells. Total RNA was purified by using RNeasy Mini kit (QIAGEN). Microarray targets from 200 ng total RNA were synthesized and labelled using the Low RNA Input Linear Amp Kit (Agilent) and hybridized to Mouse 4x44K Ver.2.0 arrays. Arrays were scanned on an Agilent Technologies Microarray scanner and signal intensities were calculated in Agilent Feature Extraction 10.7.3.1 software.

2014-07-08 | E-GEOD-59107 | biostudies-arrayexpress

Establishment of trophoblast stem cells under defined culture conditions in mice

2014-07-08 | GSE59107 | GEO

Mus musculus

Project description:CTCF binding patterns in mouse trophoblast stem cells

| PRJNA174628 | ENA

Mus musculus strain:C57BL/6

Project description:mouse neutrophils treated with mouse Myeloperoxidase-IgG

| PRJDB10969 | ENA

Mus musculus strain:C57BL/6N

Project description:Mouse sperm small RNA sequencing

| PRJDB11972 | ENA

Mus musculus

Project description:Trophoblast stem cells

| PRJNA93945 | ENA

Mus musculus

Project description:Gene expression analysis of dendritic cells treated with small chemical compounds

| PRJNA115781 | ENA

Mus musculus

Project description:Transcriptional Networks in Mouse Trophoblast Stem Cell Self-Renewal

| PRJNA118267 | ENA

Extracellular vesicle contents of mouse trophoblast cells and their predicted influence on fetal brain development

Project description:Current work characterizes small RNA within EVs derived from culture media of undifferentiated mouse trophoblast stem cells (mTSC), and mTSC differentiated into pTGCs

2024-08-28 | GSE222855 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data