Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Chromaitn immunoprecipitation of HEK293T cells trannsiently expression various proteins

PROVIDER: PRJDB9724 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR224555.fastq.gz	Fastqsanger.gz
	DRR224556.fastq.gz	Fastqsanger.gz
	DRR224557.fastq.gz	Fastqsanger.gz
	DRR224558.fastq.gz	Fastqsanger.gz
	DRR224559.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Homo sapiens

Project description:Chromatin immunoprecipitation of HEK293T cells trannsiently expression various proteins

| PRJDB10298 | ENA

Homo sapiens

Project description:Chromatin immunoprecipitation of HEK293T cells.

| PRJDB12043 | ENA

Homo sapiens

Project description:Chromatin immunoprecipitation of HEK293T cells.

| PRJDB10584 | ENA

Homo sapiens immortalized MSCs: 3A6

Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.

2010-09-25 | GSE24338 | GEO

Homo sapiens foetal mesenchymal stem cell miRNA transcriptome

Project description:We have sequenced miRNA libraries from human embryonic, neural and foetal mesenchymal stem cells. We report that the majority of miRNA genes encode mature isomers that vary in size by one or more bases at the 3’ and/or 5’ end of the miRNA. Northern blotting for individual miRNAs showed that the proportions of isomiRs expressed by a single miRNA gene often differ between cell and tissue types. IsomiRs were readily co-immunoprecipitated with Argonaute proteins in vivo and were active in luciferase assays, indicating that they are functional. Bioinformatics analysis predicts substantial differences in targeting between miRNAs with minor 5’ differences and in support of this we report that a 5’ isomiR-9-1 gained the ability to inhibit the expression of DNMT3B and NCAM2 but lost the ability to inhibit CDH1 in vitro. This result was confirmed by the use of isomiR-specific sponges. Our analysis of the miRGator database indicates that a small percentage of human miRNA genes express isomiRs as the dominant transcript in certain cell types and analysis of miRBase shows that 5’ isomiRs have replaced canonical miRNAs many times during evolution. This strongly indicates that isomiRs are of functional importance and have contributed to the evolution of miRNA genes

2014-08-06 | GSE58734 | GEO

Homo sapiens whole blood.

Project description:RNA extracted at 240min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.

2019-07-31 | GSE114179 | GEO

Homo sapiens MSCs: Normoxia vs. Hypoxia

Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.

2011-02-19 | GSE20463 | GEO

Homo sapiens

Project description:Chromatin immunoprecipitation of HEK293T cells using native and crosslinked ChIP methods.

| PRJDB10941 | ENA

Homo sapiens

Project description:ChIP-seq analysis of the HEK293T cell line transiently expressing various FLAG-tagged proteins

| PRJDB8608 | ENA

Homo sapiens

Project description:Genomic localization of MOZ mutant proteins in HEK293T cells

| PRJDB13722 | ENA