Project description:Entomopathogenic nematodes (EPNs) are gaining significant popularity as biocontrol agents due to their ability to rapidly kill insect hosts. These nematodes produce bioactive molecules known as excreted/secreted products (ESPs) that help them overcome host immune defenses. Understanding the mechanisms that trigger ESP release and their composition is crucial for unraveling the factors underlying nematode pathogenicity and enhancing their effectiveness as biocontrol agents. Leveraging the insect parasitic nematode, Heterorhabditis bacteriophora, we describe an in vitro study focused on the recovery process of infective juveniles upon contact with selected activation materials. We evaluated three host-derived materials from Galleria mellonella larvae: live larvae, frozen larvae, and heat-inactivated homogenates; and two non-insect-derived materials: filtered water and phosphate-buffered saline. While none of the tested materials resulted in 100% IJ recovery, all induced ESP release. The ESPs were collected and analyzed using liquid chromatography-tandem mass spectrometry, resulting in the identification of 372 proteins. The highest number of proteins, approximately 200, were detected in response to heat-inactivated homogenate and phosphate-buffered saline treatment. Among the identified ESPs were proteins involved in immunomodulation and protease inhibition, both crucial for overcoming host defenses. Our findings highlight the complexity of nematode-host interactions and suggest avenues for improving biocontrol strategies through a deeper understanding of nematode activation and the released spectrum of ESPs.

2025-02-10 | PXD056325 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data