Project description:The aim of this study is to identify alterations induced in gastric mucosa of mice exposed to Pteridium aquilinum and/or infected with Helicobacter pylori, in order to identify genes that are induced by bracken fern exerts exacerbating effects on gastric lesions associated to the infection. Six groups of C57Bl/6 mice were be used: 1) control, 2) infected Helicobacter pylori, 3) treated with Bracken fern extract orogastrically, 4) treated with Bracken fern extract in drinking water, 5) infected Helicobacter pylori + treated with Bracken fern extract orogastrically, 6) infected Helicobacter pylori + treated with Bracken fern extract in drinking water. The infection procedure was performed using an orogastric inoculation of H.pylori (strain SS1) twice in the first week. The RNA isolation was done in triplicate (3 mice per each condition). Further evaluation of morphological alterations on gastric mucosa, proliferative index and induction of DNA strand breaks will be performed in the mice stomach exposed to Pteridium aquilinum infected or not with Helicobacter pylori. Alterations of glycosylation in gastric tissues will also evaluated.
Project description:We have employed the Zebrafish gene expression microarray (MZH_Zebrafish_16k_v1.0) as a discovery platform to analyze the trancriptome of 108hpf (hours post fertilization) embryos exposed from the 96hpf to 108hpf to 100µM of diethylmaleate (DEM).
Project description:We have employed the Zebrafish gene expression microarray (MZH_Zebrafish_16k_v1.0) as a discovery platform to analyze the trancriptome of 108hpf (hours post fertilization) embryos exposed from the 96hpf to 108hpf to 100µM of diethylmaleate (DEM). Four two-color microarray studies for measuring expression levels of zebrafish embryos treated with 100µM of diethylmaleate (DEM) were performed. 8 samples were analyzed in total. Four samples were treated with 0.1% DMSO as a control; four samples were treated with 100 µM of DEM and 0.1% DMSO.
Project description:The aim of this study is to identify alterations induced in gastric mucosa of mice exposed to Pteridium aquilinum and/or infected with Helicobacter pylori, in order to identify genes that are induced by bracken fern exerts exacerbating effects on gastric lesions associated to the infection.
Project description:Previous study has determined Dux regulates 2CLC trancriptome via binding and activating MERVL,but the mechanism about the change happned during the 2C-exit process are rarely determined.In this study,we try to illustrate how ZFP352 binds genome location to regulate its downstream trancriptome and 2C-exit process.And make some conclusion and hypothesis about ZFP352 co-binding and interact with other trancription factor:Dux,etc to regulate 2C-exit.
Project description:Pteris cretica L var. nervosa is one of the dominent fern species at antimony mining area where arbuscular mycorrhizal fungi can be found as a symbiosis. The effect of AMF on fern exposed to long-term excessive Sb was pooly understood. The project applied this fern co-cultivting with or withour AMF under different concentration of Sb in soil for charicterising Sb phytomediation ability of it along with the effect by AMF symbiosis.
Project description:In the further study we have employed the Zebrafish gene expression microarray (v2) as a discovery platform to analysis the trancriptome of 5dpf (days post fertilization) embryos exposed from the 2dpf to 5dpf to nanomolar concrations of thyoid hormone. Only less than 5% genes were affected by the treatment. Gene ontology (GO) analyses show that these genes are involved in the haematopoietic system, visual function and skeletal system. Representatives of each GO functional groups were selected and quantified by real-time PCR to validate the microarray data and to use them as a quantitative biomarkers of thyroid receptor activator. The gene expression in Zebrafish was measured in 5 dpf's embryos under 72h Thyroid Hormone treatment at 50nM.
Project description:In the further study we have employed the Zebrafish gene expression microarray (v2) as a discovery platform to analysis the trancriptome of 5dpf (days post fertilization) embryos exposed from the 2dpf to 5dpf to nanomolar concrations of thyoid hormone. Only less than 5% genes were affected by the treatment. Gene ontology (GO) analyses show that these genes are involved in the haematopoietic system, visual function and skeletal system. Representatives of each GO functional groups were selected and quantified by real-time PCR to validate the microarray data and to use them as a quantitative biomarkers of thyroid receptor activator.