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Metagenomes from full scale mesophilic anaerobic digesters in Denmark

ABSTRACT: Metagenomes from full scale mesophilic anaerobic digesters in Denmark

PROVIDER: PRJEB10929 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1025320_1.fastq.gz	Fastqsanger.gz
	ERR1025320_2.fastq.gz	Fastqsanger.gz
	ERR1025321_1.fastq.gz	Fastqsanger.gz
	ERR1025321_2.fastq.gz	Fastqsanger.gz
	ERR1025322_1.fastq.gz	Fastqsanger.gz

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Project description:The microbial-mediated anaerobic digestion (AD) process represents an efficient biological process for the treatment of organic waste along with biogas harvest. Currently, the key factors structuring bacterial communities and the potential core and unique bacterial populations in manure anaerobic digesters are not completely elucidated yet. In this study, we collected sludge samples from 20 full-scale anaerobic digesters treating cattle or swine manure, and investigated the variations of bacterial community compositions using high-throughput 16S rRNA amplicon sequencing. Clustering and correlation analysis suggested that substrate type and free ammonia (FA) play key roles in determining the bacterial community structure. The COD: [Formula: see text] (C:N) ratio of substrate and FA were the most important available operational parameters correlating to the bacterial communities in cattle and swine manure digesters, respectively. The bacterial populations in all of the digesters were dominated by phylum Firmicutes, followed by Bacteroidetes, Proteobacteria and Chloroflexi. Increased FA content selected Firmicutes, suggesting that they probably play more important roles under high FA content. Syntrophic metabolism by Proteobacteria, Chloroflexi, Synergistetes and Planctomycetes are likely inhibited when FA content is high. Despite the different manure substrates, operational conditions and geographical locations of digesters, core bacterial communities were identified. The core communities were best characterized by phylum Firmicutes, wherein Clostridium predominated overwhelmingly. Substrate-unique and abundant communities may reflect the properties of manure substrate and operational conditions. These findings extend our current understanding of the bacterial assembly in full-scale manure anaerobic digesters.

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