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Mining for novel cellulase genes

ABSTRACT: Mining for novel cellulase genes from different ecosystem metagenomes

PROVIDER: PRJEB12864 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1294727_1.fastq.gz	Fastqsanger.gz
	ERR1294727_2.fastq.gz	Fastqsanger.gz

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Mining for novel cellulase genes metagenomic assembly

Project description:EMG produced TPA metagenomics assembly of the Mining for novel cellulase genes from different ecosystem metagenomes (Mining for novel cellulase genes) data set

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Comparative genomic, transcriptomic and secretomic profilings of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106, and identification of two novel regulators for cellulase and xylanase gene expression

Project description:In this study,comparative genomic, transcriptomic and secretomic profilings of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106 were employed to screen for novel regulators for cellulase and xylanase gene expression.

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A novel conserved transcription factor CLR-4 regulates cellulase genes expression through controlling the intracellular cAMP level in ascomycete fungi

Project description:Fungal degradation of lignocellulosic biomass requires various (hemi-)cellulases and plays key roles in biological carbon cycle. Although cellulases induction recently described in some saprobic filamentous fungi, regulation of cellulase transcription has not been studied thoroughly. Here, we identified and characterized the novel cellulase regulation factors clr-4 in Neurospora crassa and its ortholog Mtclr-4 in Myceliophthora thermophila. Deletion of clr-4 and Mtclr-4 displayed similarly defective phenotypes in cellulolytic enzymes production and activities. Transcriptomics analysis of Δclr-4/ΔMtclr-4 revealed down-regulation of not only encoding genes of (hemi-)cellulases and pivotal regulators (clr-1, clr-2 and xyr-1), but also the key genes of cAMP signaling pathway such as adenylate cyclase cr-1. Consistently, the significant decreased levels of intracellular cAMP were observed in Δclr-4/ΔMtclr-4 compared to wild-type during cellulose utilization. Electrophoretic mobility shift assays (EMSA) verified that CLR-4 could directly bind to the promoter regions of adenylyl cyclase (Nccr-1) and cellulose regulator clr-1, while MtCLR-4 bind to upstream regions of adenylyl cyclase Mtcr-1 and biomass deconstruction regulators Mtclr-2 and Mtxyr-1. Concluded, the novel cellulase expression regulators (CLR-4/MtCLR-4) findings here significantly enrich our understanding of the regulatory network of cellulose degradation and provide new targets for industrial fungi strain engineering for plant biomass deconstruction in biorefinery.

2021-03-18 | GSE111986 | GEO

Disruption of serine/threonine protein kinase STK-12 increases cellulase expression in Neurospora crassa

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The putative cellodextrin transporter-like protein CLP1 is involved in cellulase induction in Neurospora crassa

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The putative cellodextrin transporter-like protein CLP1 is involved in cellulase induction in Neurospora crassa

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2014-11-13 | E-GEOD-60004 | biostudies-arrayexpress

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