Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The genome sequence of Ramularia colly cygni URUG2

ABSTRACT: The genome sequence of Ramularia colly cygni URUG2, the barley pathogen causing RLS

PROVIDER: PRJEB12938 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	ERR2244070_1.fastq.gz	Fastqsanger.gz
	ERR2244070_2.fastq.gz	Fastqsanger.gz
	ERR2244080_1.fastq.gz	Fastqsanger.gz
	ERR2244080_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 39

Similar Datasets

Barley leaf infection with Ramularia collo-cygni

Project description:Barley plants (cv Fairytale and Tripple) were infected with two Ramularia collo-cygni isolates. Either DK05 or NZ11. Transcript and protein profiles were compared.

2024-01-24 | PXD039541 | Pride

Ramularia collo-cygni

Project description:Ramularia collo-cygni overview

| PRJNA433034 | ENA

Ramularia collo-cygni genome, DK05

Project description:De novo genome assembly and comparative genomics of the newly important barley pathogen Ramularia collo-cygni.

| PRJEB11432 | ENA

The genome of the emerging barley pathogen Ramularia collo-cygni.

Project description:BackgroundRamularia collo-cygni is a newly important, foliar fungal pathogen of barley that causes the disease Ramularia leaf spot. The fungus exhibits a prolonged endophytic growth stage before switching life habit to become an aggressive, necrotrophic pathogen that causes significant losses to green leaf area and hence grain yield and quality.ResultsThe R. collo-cygni genome was sequenced using a combination of Illumina and Roche 454 technologies. The draft assembly of 30.3 Mb contained 11,617 predicted gene models. Our phylogenomic analysis confirmed the classification of this ascomycete fungus within the family Mycosphaerellaceae, order Capnodiales of the class Dothideomycetes. A predicted secretome comprising 1053 proteins included redox-related enzymes and carbohydrate-modifying enzymes and proteases. The relative paucity of plant cell wall degrading enzyme genes may be associated with the stealth pathogenesis characteristic of plant pathogens from the Mycosphaerellaceae. A large number of genes associated with secondary metabolite production, including homologs of toxin biosynthesis genes found in other Dothideomycete plant pathogens, were identified.ConclusionsThe genome sequence of R. collo-cygni provides a framework for understanding the genetic basis of pathogenesis in this important emerging pathogen. The reduced complement of carbohydrate-degrading enzyme genes is likely to reflect a strategy to avoid detection by host defences during its prolonged asymptomatic growth. Of particular interest will be the analysis of R. collo-cygni gene expression during interactions with the host barley, to understand what triggers this fungus to switch from being a benign endophyte to an aggressive necrotroph.

| S-EPMC4979122 | biostudies-literature

Ramularia coccinea

Project description:Ramularia coccinea overview

| PRJNA636857 | ENA

BRCC R. collo-cygni and barley RNA expression

Project description:BRCC R. collo-cygni and barley RNA expression

| PRJEB14791 | ENA

Ramularia rumicis

Project description:Ramularia rumicis Genome sequencing

| PRJNA543617 | ENA

Fungicide Resistance Evolving in Ramularia collo-cygni Population in Estonia.

Project description:Ramularia leaf spot caused by the fungus Ramularia collo-cygni, has recently become widespread in Estonian barley fields. Currently, disease control in barley fields relies on SDHI and DMI fungicides, which might be threatened by R. collo-cygni isolates that are well-adapted to fungicide pressure. In a two-year study, 353 R. collo-cygni isolates were collected from spring barley fields in Estonia. A total of 153 R. collo-cygni isolates were examined for sensitivity to azoles (DMIs; prothioconazole-desthio, epoxiconazole, mefentrifluconazole) and succinate dehydrogenase inhibitors (SDHIs; boscalid, fluxapyroxad). Epoxiconazole was the least effective and a new fungicide mefentrifluconazole was the most effective DMI. Among SDHIs, fluxapyroxad was more effective than boscalid. Also, single R. collo-cygni isolates with high resistance to tested fungicides occurred, which could affect fungicide control of the pathogen. The entire collection of R. collo-cygni was analysed for mutations in fungicide target proteins. Six mutations were identified in CYP51 gene, the most dominant being I381T, I384T, and S459C. Also, numerous point mutations in the SdhC gene were present. The mutation G143A in strobilurin target protein CytB dominates in over 80% of the R. collo-cygni population, confirming the low efficacy of strobilurin fungicides in barley disease control.

| S-EPMC8307248 | biostudies-literature

A New Reference Genome Shows the One-Speed Genome Structure of the Barley Pathogen Ramularia collo-cygni.

Project description:Ramularia leaf spot has recently emerged as a major threat to barley production world-wide, causing 25% yield loss in many barley growing regions. Here, we provide a new reference genome of the causal agent, the Dothideomycete Ramularia collo-cygni. The assembly of 32 Mb consists of 78 scaffolds. We used RNA-seq to identify 11,622 genes of which 1,303 and 282 are coding for predicted secreted proteins and putative effectors respectively.The pathogen separated from its nearest sequenced relative, Zymoseptoria tritici ∼27 Ma. We calculated the divergence of the two species on protein level and see remarkably high synonymous and nonsynonymous divergence. Unlike in many other plant pathogens, the comparisons of transposable elements and gene distributions, show a very homogeneous genome for R. collo-cygni. We see no evidence for higher selective pressure on putative effectors or other secreted proteins and repetitive sequences are spread evenly across the scaffolds. These findings could be associated to the predominantly endophytic life-style of the pathogen. We hypothesize that R. collo-cygni only recently became pathogenic and that therefore its genome does not yet show the typical pathogen characteristics. Because of its high scaffold length and improved CDS annotations, our new reference sequence provides a valuable resource for the community for future comparative genomics and population genetics studies.

| S-EPMC6301796 | biostudies-literature

Expression profiles of Ramularia collo-cygni (strain urug 2) grown on 6 conditions revealed common and different sets of regulated genes

Project description:The fungal RNA from 6 different conditions, especially one that mimics the plant environment (Barley Straw Agar –BSagar) was also sequenced to help in one hand the annotation and in other hand to uncover putative gene of interest that might be involved in the pathogenicity or the fungicide resistances for example.

2018-01-13 | GSE109135 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data