Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Apoplastic H2O2 effects on phenolic metabolism and gene expression in a Norway spruce lignin-forming cell culture

PROVIDER: PRJEB13079 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR1331750_1.fastq.gz	Fastqsanger.gz
	ERR1331750_2.fastq.gz	Fastqsanger.gz
	ERR1331760_1.fastq.gz	Fastqsanger.gz
	ERR1331760_2.fastq.gz	Fastqsanger.gz
	ERR1331751_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Proteomics of exovesicle-enriched fraction of Norway spruce

Project description:Exovesicle-enriched fraction was isolated from the culture medium of tissue-cultured cells of Norway spruce during extracellular lignin formation and when lignin formation was hindered by scavenging of H2O2 from the culture medium. Proteomic analysis was conducted to investigate the protein cargo of the exovesicles.

2023-05-16 | MSV000091958 | MassIVE

Proteomics of exovesicle-enriched fraction of Norway Spruce, non-EDTA-extracted samples

2023-06-30 | MSV000092312 | MassIVE

Down-regulation of a gene encoding CCR affects... cell wall function in embryogenic cultures of Norway spruce

Project description:An attractive objective in the breeding of forest trees is to reduce the content of lignin or alter the lignin composition. However, for long-lived forest trees, such as conifers, it is time consuming and expensive to wait until mature wood is formed for estimating how different ligninfication genes affect cell wall differentiation. The aim of this work was to examine the possibility of detecting changes in global gene expression and of genes involved in lignin biosynthesis already in embryogenic cultures carrying the native Norway spruce cinnamoyl CoA reductase (CCR) gene in antisense orientation. Several genes, including genes regulating phenylalanine ammonia-lyase and peroxidase, regulating different pathways associated with lignin biosynthesis were affected but the transcript abundances of genes regulating lignin biosynthesis were not affected. Functional assignment defined by Gene Ontology terms, which provide functional classification for genes and gene products representing their corresponding biological process, cellular component and molecular function showed that transformation with asCCR affects a broad spectrum of functional categories, especially processes taking place in the cell wall. Furthermore, many changes in genes related to chromatin, DNA and chromosome take place. Keywords: transgenic Norway spruce, embryogenic culture

2006-12-27 | GSE6524 | GEO

Hunting monolignol transporter(s): membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce

Project description:Monolignol transport during lignification is a partially solved puzzle: both the mechanism(s) and the transported form of monolignols are unknown in developing xylem of trees. We tested a hypothesis of an active, plasma membrane (PM)-localized transport of monolignol monomers, dimers, and/or glucosidic forms with membrane vesicles prepared of developing xylem, phloem, and lignin-forming, tissue-cultured cells of Norway spruce (Picea abies L. Karst.), as well as of a control material, non-lignifying tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported both coniferin and p-coumaryl alcohol glucoside, but inhibitor assays suggested this transport being over the tonoplast. Based on similar inhibitor assays, lignin-forming, tissue42 cultured cells of spruce had coniferin transport putatively localizing on PM. Liquid chromatography-mass spectrometry (LC-MS/MS) analysis of membrane proteins isolated from spruce developing xylem, phloem and tissue-cultured cells revealed multiple transporters. These were compared to a transporter gene set that was gained by a correlation analysis with a selected set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays showed no support for the ABC-transporter-mediated monolignol transport but point to secondary active transporters (such as MFS- or MATE-transporters). In contrast, proteomic and co-expression analyses suggest a role for ABC-transporters and MFS-transporters.

2020-08-14 | PXD017533 | Pride

Proteomic analysis of unconcentrated exovesicle-depleted cell culture medium of Norway spruce

Project description:Exovesicle-enriched fraction was isolated from the culture medium of tissue-cultured cells of Norway spruce during extracellular lignin formation by an ultracentrifugation-based protocol. From the remaining, now exovesicle-depleted culture medium, 11.8 ul of the medium was prepared for proteomic analysis.

2023-12-18 | MSV000093681 | MassIVE

DNA methylome study of Norway spruce

Project description:Bisulfite treatment libraries were made to characterize DNA methylation of Norway spruce

2016-11-22 | GSE86983 | GEO

PARE libraries for the identification of miRNA target genes in Norway spruce

Project description:We constructed four PARE libraries for the identification of miRNA target genes in Norway spruce.

2015-09-02 | GSE65248 | GEO

Norway Spruce Cultured Cells Chitosan & C.polonica Stress

Project description:Conifer-specific responses to elicitation with the chemical elicitor chitosan have been investigated using a transcriptome analysis in Norway spruce using a cell suspension culture system that has been previously described (Phillips, Walter et al. 2007). This study has demonstrated that the early events following chitosan elicitation include calcium mediated signaling and an oxidative response that have not previously been described in intact trees. Keywords: stress response

2008-06-01 | GSE10771 | GEO

MdMYB52 regulates lignin biosynthesis upon the suberization process in apple.

Project description:Our previous studies, comparing russeted vs. waxy apple skin, highlighted a MYeloBlastosys (Myb) transcription factor (MdMYB52-like), which displayed a correlation with genes associated to the suberization process. The present article aims to assess its role and function in the suberization process. Phylogenetic analyses and research against Arabidopsis thaliana MYBs database were first performed and the tissue specific expression of MdMYB52-like was investigated using RT-qPCR. The function of MdMYB52-like was further investigated using Agrobacterium-mediated transient overexpression in Nicotiana benthamiana leaves. An RNA-Seq analysis was performed to highlight differentially regulated genes in response MdMYB52-like. Transcriptomic data were supported by analytical chemistry and microscopy. A massive decreased expression of photosynthetic and primary metabolism pathways was observed with a concomitant increased expression of genes associated with phenylpropanoid and lignin biosynthesis, cell wall modification and senescence. Interestingly key genes involved in the synthesis of suberin phenolic components were observed. The analytical chemistry displayed a strong increase in the lignin content in the cell walls during MdMYB52 expression. More specifically, an enrichment in G-Unit lignin residues was observed, supporting transcriptomic data as well as previous work describing the suberin phenolic domain as a G-unit enriched lignin-like polymer. The time-course qPCR analysis revealed that the observed stress response, might be explain by this lignin biosynthesis and by a possible programmed senescence triggered by MdMYB52-like. The present work supports a crucial regulatory role for MdMYB52 in the biosynthesis of the suberin phenolic domain and possibly in the fate of suberized cells in russeted apple skins.

2022-09-26 | GSE212828 | GEO

Project description:Are lignin monomers secreted from ray parenchymal cells in lignifying norway spruce xylem?

| PRJEB10305 | ENA