Project description:Screen for differences in gene expression between a parental Salmonella enterica serovar Enteritidis strain (ATCC4931) and an adapted strain with increased resistance to the widely used antimicrobial sanitizer dodecyltrimethylammonium chloride (DTAC)
Project description:White leghorn layers were infected with Salmonella Enteritidis. The cecum were collected at 7 days post infection for total RNA isolation. The significantly expressed microRNAs between infected and non-infected chickens were identified through Solexa sequencing technology.
Project description:Investigation of whole genome gene expression level changes in Salmonella enterica serova Enteritidis and Typhimurium under chlorine treatment
Project description:Screen for differences in gene expression between a parental Salmonella enterica serovar Enteritidis strain (ATCC4931) and an adapted strain with increased resistance to the widely used antimicrobial sanitizer dodecyltrimethylammonium chloride (DTAC) Time course of comparative gene expression changes between log phase parental and adapted Enteritidis strains after 0, 10, 30 and 150 min of exposure to 50% of the respective MIC of DTAC.
Project description:Salmonella Entertidis (SE) causes persistent infections and egg contamination in laying ducks.Hcp roles as the core structural and effector proteins of T6SS. We generated an hcp deletion mutant MY1△hcp and detected its ability to invade duck granulosa cells (dGCs) and contaminate eggs. Then, Quantitative proteomics of Hcp-mediated Salmonella Enteritidis was performed.
Project description:Salmonella enterica subsp. enterica contains more than 2,600 serovars of which four are of major medical relevance for humans. While the typhoidal serovars (Typhi and Paratyphi A) are human-restricted and cause enteric fever, non-typhoidal Salmonella serovars (Typhimurium and Enteritidis) have a broad host range and predominantly cause gastroenteritis. In this study, we compared the core proteomes of Salmonella Typhi, Paratyphi A, Typhimurium and Enteritidis using contemporary proteomics. Five isolates, covering different geographical origins, and one reference strain per serovar were grown in vitro to the exponential phase. Protein levels of orthologous proteins between serovars were compared and subjected to gene ontology term enrichment and inferred regulatory interactions. Differential expression of the core proteomes of the typhoidal serovars appears mainly related to cell surface components and, for the non-typhoidal serovars, to pathogenicity. Our findings may guide future development of novel diagnostics and vaccines, and understanding of disease progression.
Project description:We report our results of RNA-seq analysis on the wild type Salmonella Enteritidis and its yccT (i.e., yccT encodes STY1099 protein) mutant with and without H2O2 treatment
Project description:The screening of a cDNA derived expression library of Salmonella Enteritidis 125109 expressed in E. coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. Thus, 1536 different clones were screened including positive (fimA) and negative (argC, gapA) reference proteins. The goal of the screening was to identify potential novel immunogenic proteins from S. Enteritidis by selecting clones showing a high signal intensity in comparison to the known antigens used as positve markers. Afterwards, the most promising clones were sequenced to identify the gene and corresponding protein and these proteins were then investigated further. Consequently, 9 novel immunogenic proteins could be identified.
