Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Transcriptome sequencing reveals novel candidate genes for Cardinium hertigii-caused cytoplasmic incompatibility

PROVIDER: PRJEB13864 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR1397970.fastq.gz	Fastqsanger.gz
	ERR1397971.fastq.gz	Fastqsanger.gz
	ERR1397966.fastq.gz	Fastqsanger.gz
	ERR1397967.fastq.gz	Fastqsanger.gz
	ERR1397968.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Candidatus Cardinium hertigii

Project description:Candidatus Cardinium hertigii overview

| PRJNA248243 | ENA

Candidatus Cardinium hertigii strain:cHgTN10

Project description:Candidatus Cardinium hertigii strain:cHgTN10 Assembly

| PRJNA471768 | ENA

Culicoides biting midge endosymbionts

Project description:Assessing the Phylogeographic Distribution of Candidatus Cardinium hertigii in Culicoides

| PRJEB39910 | ENA

Candidatus Cardinium hertigii strain:Zchori-Fein et al. 2004

Project description:Cardinium hertigii Zchori-Fein et al. 2004 genome sequencing project

| PRJEA66421 | ENA

Comparative analysis of stylar canal cells transcriptome in clementine mandarin (Citrus clementina)

Project description:Compared to what is known in model species, reproductive biology in citrus is still poorly understood. Although in recent years several efforts have been made to study pollen-pistil interaction and self-incompatibility, little information is available about the molecular mechanisms regulating these processes. We performed microarray analysis for the identification of candidate genes involved in pollen-pistil interaction and self-incompatibility in clementine (Citrus clementina Hort. ex Tan.). The analysis was performed comparing the transcriptome of laser-microdissected stylar canal cells isolated from two clementine genotypes differing for self-incompatibility response (‘Comune’, self-incompatible; and ‘Monreal’, a self compatible mutation of ‘Comune’).

2012-02-15 | GSE33014 | GEO

Genome of Candidatus Cardinium hertigii cBtQ1

Project description:Genome of Candidatus Cardinium hertigii endosymbiont of Bemisia tabacia, strain cBtQ1

| PRJEB4234 | ENA

Spiroplasma endosymbiont of Lariophagus distinguendus isolate:STU

Project description:Genomics of Spiroplasma-induced cytoplasmic incompatibility

| PRJNA826431 | ENA

cidA and cidB genes repertoires an CI

Project description:Culex pipiens mosquitoes cidA and cidB genes repertoires and Cytoplasmic Incompatibility

| PRJEB35266 | ENA

insect metagenome

Project description:A novel clade of alphaproteobacterial endosymbionts induces complete cytoplasmic incompatibility in the coconut beetle

| PRJDB5723 | ENA

The relative importance of DNA methylation and Dnmt2-mediated epigenetic regulation on Wolbachia densities and cytoplasmic incompatibility

Project description:Wolbachia pipientis is a worldwide bacterial parasite of arthropods that infects host germline cells and manipulates host reproduction to increase the ratio of infected females, the transmitting sex of the bacteria. The most common reproductive manipulation, cytoplasmic incompatibility (CI), is expressed as embryonic death in crosses between infected males and uninfected females. Specifically, Wolbachia modify developing sperm in the testes by unknown means to cause a post-fertilization disruption of the sperm chromatin that incapacitates the first mitosis of the embryo. As these Wolbachia-induced changes are stable, reversible, and affect the host cell cycle machinery including DNA replication and chromosome segregation, we hypothesized that the host methylation pathway is targeted for modulation during cytoplasmic incompatibility because it accounts for all of these traits. Here we show that infection of the testes is associated with a 55% increase of host DNA methylation in Drosophila melanogaster, but methylation of the paternal genome does not correlate with penetrance of CI. Overexpression and knock out of the Drosophila DNA methyltransferase Dnmt2 neither induces nor increases cytoplasmic incompatibility. Instead, overexpression decreases Wolbachia titers in host testes by approximately 17%, leading to a similar reduction in CI levels. Finally, strength of CI induced by several different strains of Wolbachia does not correlate with levels of DNA methylation in the host testes. We conclude that DNA methylation mediated by Drosophila's only known methyltransferase is not required for the transgenerational sperm modification that causes CI. Genomic DNA was extracted from pooled samples of Drosophila melanogaster adult testes. One sample from Wolbachia-infected males and one from uninfected males. Bisulfite sequencing was used to determine whether Wolbachia infection affects host DNA methylation in the testes.

2014-12-03 | E-GEOD-63795 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data