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Functional implications of miR-19 in the migration of newborn neurons in the adult brain

ABSTRACT: Functional implications of miR-19 in the migration of newborn neurons in the adult brain

PROVIDER: PRJEB14394 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1450590.fastq.gz	Fastqsanger.gz
	ERR1450600.fastq.gz	Fastqsanger.gz
	ERR1450591.fastq.gz	Fastqsanger.gz
	ERR1450601.fastq.gz	Fastqsanger.gz
	ERR1450592.fastq.gz	Fastqsanger.gz

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Functional implications of miR-19 in the migration of newborn neurons in the adult brain

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Project description:Zebrafish display widespread and pronounced adult neurogenesis, which is fundamental for their regeneration capability after central nervous system injury. However, the cellular identity and the biological properties of adult newborn neurons are elusive for most brain areas. Here, we used short-term lineage tracing of radial glia progeny to prospectively isolate newborn neurons from the her4.1+ radial glia lineage in the homeostatic adult forebrain. Transcriptome analysis of radial glia, newborn neurons and mature neurons using single cell sequencing identified distinct transcriptional profiles including novel markers for each population. Specifically, we detected 2 separate newborn neuron types, which showed diversity of cell fate commitment and location. Further analyses showed homology of these cell types to neurogenic cells in the mammalian brain, identified neurogenic commitment in proliferating radial glia and indicated that glutamatergic projection neurons fate are generated in the adult zebrafish telecephalon. Thus, we prospectively isolated adult newborn neurons from the adult zebrafish forebrain, identified markers for newborn and mature neurons in the adult brain, revealed intrinsic heterogeneity among adult newborn neurons and their homology to mammalian adult neurogenic cell types.

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