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Campylobacter jejuni whole genome shot-gun sequencing

ABSTRACT: Application of whole-genome sequencing for surveillance of food-borne pathogen Campylobacter jejuni

PROVIDER: PRJEB15115 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1579000_1.fastq.gz	Fastqsanger.gz
	ERR1579000_2.fastq.gz	Fastqsanger.gz
	ERR1579010_1.fastq.gz	Fastqsanger.gz
	ERR1579010_2.fastq.gz	Fastqsanger.gz
	ERR1579001_1.fastq.gz	Fastqsanger.gz

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Campylobacter jejuni transcriptomic response to benzylisothiocyanate

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Project description:This study investigates the RNA targets and cleavage sites of endogenous Cas9 in the food-borne pathogen Campylobacter jejuni. Direct RNA binding targets of Cas9 in C. jejuni strain NCTC11168 were determined using RIP-seq. The Cleavage sites were then predicted in the RNA targets by comparing total transcriptome data from WT and deletion (cas9, crRNA3, tracrRNA, CRISPR-tracrRNA) strains. PAMs for the CjeCas9 were enriched using the PAM-SCANR platform, which operates through a GFP reporter gene. Upon GFP (and thus functional PAM) enrichment, fluorescing cells were isolated using FACS and prepared plasmid DNA was amplified and prepared for sequencing.

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