Project description:In this study, we evaluated the protein expression changes in multidrug-resistant P. aeruginosa and C. albicans treated with recombinant defensin-d2 and actifensin from spinach and Actinomyces ruminicola, respectively, for 1 h. The proteome changes in the test organisms were analyzed by LC-MS/MS ESI using label-free data-dependent acquisition (DDA) quantitative proteomics technique. A total of 28 and 9 differentially expressed proteins (DEPs) were identified in the treated P. aeruginosa and C. albicans, respectively, with a 2-fold change threshold and p-value < 0.05. The GO and KEGG enrichment analysis found that DEPs related to binding, catalytic activity, cellular component organization and biogenesis, structural molecule activity and response to stimulus were significantly expressed in actifensin and defensin-treated P. aeruginosa, and the DEPs were mainly involved in DNA replication and repair, translation, membrane transport, energy metabolism, environmental adaptation, and signal transduction. DEPs in actifensin and defensin-treated C. albicans were related to cell growth and death, translation, lipid metabolism and energy metabolism were significantly expressed, and these DEPs mainly involved oxidative phosphorylation, RNA degradation, carbohydrate metabolism, and cell cycle pathways. We also established evidently through the different pathways affected, that the recombinant peptides exerted multiple mechanisms of action against the test organisms, sequentially or simultaneously.
Project description:Using 270K Nimblegen Comparative Genomic Hybridization (CGH) array on a set of cv. Chinese Spring deletion lines, a total of 3,671 sequence contigs and scaffolds (~7.8% of chromosome 7B physical length) were mapped into nine deletion bins. In our study we have developed 270K CGH Nimblegen array containing wheat 7B chromosome specific probes and genotyped wheat 7B deletion stocks which have terminal deletions in 7B. Our main aim was to identify absent probes (sequences) in deletion lines. Initially, spatial normalization and M-A loess normalization was performed for comparison test/reference and then clustering analysis (Mcust) was carried out. Further analysis was done on scaffolds (i.e. on larger sequences instead of probes; probes are designed from scaffolds)
