Project description:We evaluated the possible mechanisms by which exposure to a sequentially treated pulp and paper mill effluent affects gene expression in the liver of male and female fathead minnows.
2012-12-31 | GSE25928 | GEO
Project description:Bacteria and Archaea ecology of Palm Oil Mill Effluent Raw sequence reads
Project description:Oil palm (Elaeis guineensis) is a highly productive oil crop and the most consumed vegetable oil globally due to its unique oil characteristics. Palm oil production is affected by abiotic stresses, leading to food security issues and huge economic losses. To understand the oil palm responses to different abiotic stresses at the transcriptional level, we performed RNA-Seq analyses of oil palm leaves treated with drought, high salinity, heat, cold and flood stresses compared with controls. A total of 18 libraries (three biological replicates per treatment) were generated with approximately 843 million of total clean reads after data filtering. Clean reads were mapped to the oil palm reference genome at a total mapping rate of more than 70%. The quantification of expression analysis enabled the identification of potential abiotic stress-responsive genes, co-expressed under multiple abiotic stresses and unigenes that were induced by a specific abiotic stress. The availability of these RNA-Seq datasets will provide a better understanding of the abiotic stress response mechanisms to develop climate-resilient oil palm planting materials.
Project description:We evaluated the possible mechanisms by which exposure to a sequentially treated pulp and paper mill effluent affects gene expression in the liver of male and female fathead minnows. Sexually mature fathead minnows were exposed to either river water, which served as our control (C), 10% untreated kraft effluent (UTK), 25% treated kraft effluent (TK) or 100% final effluent (CMO) from a multiprocess pulp and paper mill for 6 days. A total of 4 treatments. Each exposure aquarium consisted of a 42.1 L column that contained individual 5.3 L chambers. Each chamber contained a FHM breeding pair. A total of 3 biological replicates for male and female FHM per treatment were sent for microarray analysis resulting in a total of 24 arrays run as a reference design with a pooled sample of the 6 river water exposed fish serving as the reference sample..