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Calu6 and LoVo xenograft mRNA expression profiles in response to cediranib treatment

ABSTRACT: Calu6 and LoVo xenograft mRNA expression profiles in response to cediranib treatment

PROVIDER: PRJEB1858 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR266239.fastq.gz	Fastqsanger.gz
	ERR266240.fastq.gz	Fastqsanger.gz
	ERR266241.fastq.gz	Fastqsanger.gz
	ERR266242.fastq.gz	Fastqsanger.gz
	ERR266243.fastq.gz	Fastqsanger.gz

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Calu6 and LoVo xenograft mRNA expression profiles in response to cediranib treatment

Project description:Calu6 and LoVo xenograft mRNA expression profiles in response to cediranib treatment

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Project description:We sought to elucidate functions of LIN28B and potential mechanisms whereby it may promote metastasis by comparing the gene expression profile of LIN28B metastases to primary tumors. Accordingly, we performed microarray analysis on total RNA isolated from empty vector tumors, LIN28B-LoVo tumors, and LIN28B-LoVo metastases Constitutive LIN28B expression was achieved in the LoVo (ATCC #CCL-229) colon cancer cell line via retroviral transduction of MSCV-PIG-LIN28B. xenografts were produced via injection of 1x106 cells subcutaneously into the rear flanks of nude mice. Microarrays were conducted on primary tumors from empty vector and LIN28B-expressing cells, as well as metastases derived from primary tumors constitutively expressing LIN28B. (Note: metastases did not occur with empty vector tumors)

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Expression data from Irinotecan resistant LoVo cells comapred to parental LoVo cells

Project description:Irinotecan, an analogue of camptothecin, is frequently used in combination with various anticancer drugs or as a single agent in treatment of colorectal cancer. But drug resistance of tumor is still a major obstacle to overcome for the success of cancer treatment. In this study, We established chronic irinotecan resistant cell line for new marker to increase the sensitivity to irinotecan and investigated gene expression profiles of the irinotecan-resistant colorectal cancer cell line. To create stable CRC cell line chronically resistant to Irinotecan, LoVo cell was exposed to an initial Irinotecan concentration of 0.1 M-NM-<mol/L in RPMI 1640 supplemented with 10% FBS. When the growth of the cultured cells reaches at 80% confluency, cells were passaged twice at same drug concentration to ensure adaptation and then concentration of Irinotecan was sequentially increased in the same manner to 8 M-NM-<mol/L and then we investigated the gene expressions between parental colorectal cancer cell line, LoVo and Irinotecan resistant LoVo cell lines

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