Project description:Arthrospira platensis C1, a filamentous cyanobacterium, is one of the most commercially successful microalgae. However, high temperature stress is a critical problem that alters metabolic functions and causes irreversible damage during the growth of microorganisms, resulting in a loss of biomass productivity and high-value compounds. The investigation of genome-wide expression changes can provide towards a better understanding on the cellular stress responses.
Project description:For the filamentous cyanobacterium Anabaena variabilis to grow without combined nitrogen, certain cells differentiate into heterocysts that fix N2, while vegetative cells perform photosynthesis. Much remains unknown on how heterocysts differ from vegetative cells in terms of carbon and energy metabolisms. Microarrays were used to investigate gene transcription patterns in vegetative cells, heterocysts, and filaments of N2-fixing phototrophic, mixotrophic, and heterotrophic cultures.
Project description:Cappable-Seq (Ettwiller et. al., BMC Genomics volume 17, Article number: 199 (2016)) was employed to identify transcriptional start sites (TSSs) representing the primary transcriptome of hormogonia, motile filaments of the filamentous cyanobacterium in N. punctiforme ATCC29133. TSSs were defined using the wild-type strain and subsequently the expression of these TSSs was analyzed in each of three sigma-factor deletion mutant strains.
