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TGF-b2 regulates Theileria annulata gene transcription

ABSTRACT: TGF-b2 drives catalase transcription to regulate tumor H2O2 levels, and Theileria gene expression in transformed macrophages

PROVIDER: PRJEB19381 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1829140_1.fastq.gz	Fastqsanger.gz
	ERR1829140_2.fastq.gz	Fastqsanger.gz
	ERR1829141_1.fastq.gz	Fastqsanger.gz
	ERR1829141_2.fastq.gz	Fastqsanger.gz
	ERR1829142_1.fastq.gz	Fastqsanger.gz

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Novel roles for GZMA and RASGRP1 in suppressing dissemination of both Theileria annulata-transformed macrophages and human B-lymphoma cells

Project description:Theileria annulata is a tick-transmitted apicomplexan parasite that infects and transforms bovine leukocytes into disseminating tumors that cause a disease called tropical theileriosis. Using comparative transcriptomics we identified genes transcriptionally perturbed during Theileria-induced transformation and highlighted a small set of genes associated with leukocyte dissemination. CRISPR/Cas9-mediated knock-down of GZMA and RASGRP1 in macrophages attenuated for dissemination led to a regain in their dissemination in Rag2/gC mice confirming their suppressor roles in vivo. Comparing the transcriptomes of 934 human cancer cell lines to that of Theileria-transformed bovine B cells again highlighted GZMA and RASGRP1 and CRISPR-mediated overexpression of GZMA and RASGRP1 dampened the dissemination potential of human B-lymphomas. The ensemble provide evidence for a novel suppressor function in the dissemination of both T. annulata-transformed bovine leukocytes and human B-lymphomas.

2020-08-18 | GSE135377 | GEO

Cell cycle dependent phosphorylation of transmembrane proteins on the Theileria annulata schizont surface

Project description:The invasion of Theileria sporozoites into bovine leukocytes is rapidly followed by the liberation of the parasite from the parasitophorpous vacuole membrane, allowing the parasite to establish its niche within the host cell cytoplasm. Theileria infection induces host cell transformation, characterised by increased host cell proliferation and invasiveness, and the activation of anti-apoptotic genes. This process is strictly dependent on the presence of a viable parasite. Several host cell kinases, including PI3-K, PKA, CK2 and Src-family kinases, are known to be constitutively activated in Theileria infected cells and to contribute to the transformed phenotype. A number of host cell molecules, including the IB kinase and polo-like kinase 1 (Plk1), are recruited to the schizont surface, although very little is known about the schizont molecules involved. In this study we used immunofluorescence analysis to detect phosphorylated threonine (p-Thr and p-Thr-Pro) and serine (p-Ser) epitopes on the schizont during host cell cycle progression, revealing extensive schizont surface phosphorylation during host cell interphase. Further, we isolated schizonts from infected macrophages following synchronisation in S-phase or mitosis, and used mass spectrometry to detect phosphorylated schizont proteins. In total, 65 phosphorylated Theileria proteins were detected, 15 of which are potentially secreted or expressed on the surface of the schizont and thus mayight be targets forof host cell kinases. In particular, we describe the cell-cycle stage phosphorylation of two T. annulata surface expressed proteins, TaSP and p104. Phospho-peptides of both proteins were significantly more abundant in schizonts purified from S-phase cells.

2014-12-12 | PXD000899 | Pride

Theileria annulata

Project description:ApiAP2 Factors as Candidate Regulators of Stochastic Commitment to Merozoite Production in Theileria annulata

| PRJNA291177 | ENA

A bovine lymphosarcoma cell line infected with Theileria annulata exhibits an irreversible reconfiguration of host cell gene expression

Project description:The experiment investigates bovine gene expression in response to BW720c treatment in uninfected and Theileria annulata-infected cell cultures Theileria annulata, an intracellular parasite of bovine lymphoid cells, induces substantial phenotypic alterations to its host cell including continuous proliferation, cytoskeletal changes and resistance to apoptosis. While parasite induced modulation of host cell signal transduction pathways and NFκB activation are established, there remains considerable speculation on the complexities of the parasite directed control mechanisms that govern these radical changes to the host cell. Our objectives in this study were to provide a comprehensive analysis of the global changes to host cell gene expression with emphasis on those that result from direct intervention by the parasite. By using comparative microarray analysis of an uninfected bovine cell line and its Theileria infected counterpart, in conjunction with use of the specific parasitacidal agent, buparvaquone, we have identified a large number of host cell gene expression changes that result from parasite infection. Our results indicate that the viable parasite can irreversibly modify the transformed phenotype of a bovine cell line. 50% of genes with altered expression failed to show a reversible response to parasite death, a possible contributing factor to initiation of host cell apoptosis. The genes that did show an early predicted response to loss of parasite viability highlighted a sub-group of genes that are likely to be under direct control by parasite infection. Network and pathway analysis demonstrated that this sub-group is significantly enriched for genes involved in regulation of expression and chromatin modification. The results provide evidence that the Theileria parasite has the regulatory capacity to generate widespread change to host cell gene expression in a complex and reversible manner.

2013-07-04 | GSE44414 | GEO

Modulation of activation-associated host cell gene expression by the apicomplexan parasite Theileria annulata

Project description:The experiment investigates bovine gene expression in response to LPS in uninfected and Theileria annulata-infected cell cultures A subset of genes are identified which are activated in response to LPS stimulation with further modulation due to parasite infection.

2012-10-01 | GSE36428 | GEO

Gene expression in untreated and TGF-b2-treated human bone marrow-derived mesenchymal stem cells

Project description:To investigate the transcriptome level changes in human bone marrow-derived mesenchymal stem cells following treatment with TGF-b2, we used RNA-sequencing to compare gene expression between cells from the same donors cultured in standard media or media containing 1 ng/ml TGF-b2.

2023-01-30 | GSE220294 | GEO

Gene expression in untreated and TGF-b2-treated equine bone marrow-derived mesenchymal stem cells

Project description:To investigate the transcriptome level changes in gene expression in equine mesenchymal stem cells following treatment with TGF-b2, we used RNA-sequencing to compare gene expression between mesenchymal stem cells from the same donor horses cultured in standard media and media containing TGF-b2.

2022-07-06 | GSE207394 | GEO

miRNA profiling of extracellular vesicles from Theileria annulata infected B cells (TBL20) and their uninfected control counterparts (BL20)

Project description:We report significant changes in six bovine miRNA in vesicles from Theileria annulata infected cells

2019-05-14 | GSE118456 | GEO

Theileria annulata TaHDAC1

Project description:Theileria annulata histone deacetylase 1 (TaHDAC1) regulates schizont to merozoite stage conversion

| PRJEB50801 | ENA

Theileria annulata Ta9

Project description:Effect of secreted Theileria annulata effector protein Ta9 on bovine macrophage gene expression

| PRJEB77601 | ENA