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Single cell RNA-sequencing of Spike-ins and mESC using Smart-Seq2 on 96-well plates

ABSTRACT: Single cell RNA-sequencing of Spike-ins and mESC using Smart-Seq2 on 96-well plates

PROVIDER: PRJEB19995 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR1883130_1.fastq.gz	Fastqsanger.gz
	ERR1883130_2.fastq.gz	Fastqsanger.gz
	ERR1883140_1.fastq.gz	Fastqsanger.gz
	ERR1883140_2.fastq.gz	Fastqsanger.gz
	ERR1883150_1.fastq.gz	Fastqsanger.gz

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Single cell RNA-sequencing of Spike-ins and mESC using Smart-Seq2 on 96-well plates

Project description:In this study, we assess technical differences between commonly used single-cell RNA-Sequencing (scRNA-Seq) methods. We perform scRNA-seq on a homogenous population of mouse embryonic stem cells along with two kinds of control spike-in molecules to assess sensitivity and accuracy of these specific methods. In this dataset, we assess the RNA-degradation and decay rates by subjecting both spike-in molecules to range of repeated freezing and thawing (freeze-thaw) cycles. We manually add spike-in molecules across a 96-well plate (containing cells and reagents), perform Smart-Seq2 method manually and generate single-cell libraries using Nextera XT kit

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Single cell RNA-sequencing of Spike-ins and mESC using Smart-Seq2 on C1 System

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