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3' RNA-seq of CD142+/- human adipose stem and progenitor cells

ABSTRACT: 3' RNA-seq of CD142+/- human adipose stem and progenitor cells

PROVIDER: PRJEB21181 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1999980.fastq.gz	Fastqsanger.gz
	ERR1999990.fastq.gz	Fastqsanger.gz
	ERR1999981.fastq.gz	Fastqsanger.gz
	ERR1999991.fastq.gz	Fastqsanger.gz
	ERR1999982.fastq.gz	Fastqsanger.gz

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3' RNA-seq of CD142+/- mouse adipose stem and progenitor cells

Project description:To characterize the newly discovered Areg cells, we sampled at least four replicates of mouse adipose stem and progenitor cells (ASPCs), CD142+ ASPCs and CD142- ASPCs at four distinct time-points: (1) immediately post-sort (D0), (2) after attachment (5 hours after plating, 5H), (3) after culturing (24 hours after plating, D1) and (4) after adipogenic differentiation (day 12 after plating, day 8 after induction of differentiation, D12), and investigated their transcriptome through a highly multiplexed 3 polyA-selective mRNA-sequencing assay.

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3' RNA-seq of CD142+/- mouse adipose stem and progenitor cells

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Project description:To validate that the robustness of Aregs' (CD142+ ASPCs') molecular identity regardless of the antibody used, we performed transcriptomic profiling of freshly isolated CD142− and CD142+ mouse adipose stem and progenitor cells (ASPCs) sorted using four different anti-CD142 antibodies. ASPCs were collected as Lin− (CD31− CD45− TER119−) CD29+ CD34+ SCA1+ CD142+/− cells of the mouse subcutaneous stromal vascular fraction using FACS.

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