Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chara RNAseq

ABSTRACT: Transcriptomes of LIGHT and DARK incubated Chara australis internodal cells

PROVIDER: PRJEB21458 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2013965_1.fastq.gz	Fastqsanger.gz
	ERR2013965_2.fastq.gz	Fastqsanger.gz
	ERR2013966_1.fastq.gz	Fastqsanger.gz
	ERR2013966_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Chara membrane proteome - Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells

Project description:Membrane fractions from Chara australis internodal cells were prepared by differential centrifugation. Chara cells were incubated in normal light/dark cycles or in the dark to obtain chaasome-enriched and depleted sample, respectively. Additionally, acidic and alkaline bands of individual Chara cells were separated for membrane preparations, too.

2018-07-19 | PXD006785 | Pride

Chara vulgaris

Project description:Chara vulgaris

| PRJNA19811 | ENA

Chara corallina

Project description:Chara corallina overview

| PRJNA158205 | ENA

Chara braunii

Project description:Chara braunii overview

| PRJNA496240 | ENA

Chara corallina

Project description:Transcriptome of Chara corallina

| PRJNA606121 | ENA

Chara braunii

Project description:Chara braunii genome sequencing project

| PRJDB3348 | ENA

High-throughput sequencing of small RNAs in Chara corallina

Project description:Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from Chara corallina thallus in different growing conditions. The high depth of the resulting datasets enabled us to examine in detail critical small RNA features, such as size distribution, tissue-specific regulation and sequence conservation between different organs in this species. We also developed database resources and a dedicated website (http://smallrna.udel.edu/) with computational tools for allowing other users to identify new miRNAs or siRNAs involved in specific regulatory pathways, verify the degree of conservation of these sequences in other plant species and map small RNAs on genes or larger regions of the maize genome under study. Small RNA libraries were derived from thallus of Chara corallina incubated in different growth conditions. Total RNA was isolated using the Plant RNA Purification Reagent (Invitrogen) and submitted to Illumina (Hayward, CA, http://www.illumina.com) for small RNA library construction using approaches described in (Lu et al., 2007) with minor modifications. The small RNA libraries were sequenced with the Sequencing-By-Synthesis (SBS) technology by Illumina. PERL scripts were designed to remove the adapter sequences and determine the abundance of each distinct small RNA. We thank John Boyer for providing the plant material, as well as Kan Nobuta and Gayathri Mahalingam for assistance with the computational methods.

2011-04-29 | E-GEOD-28752 | biostudies-arrayexpress

Chara vulgaris

Project description:Analysis of expressed genes from Chara vulgaris

| PRJNA79977 | ENA

Project description:Chara australis transcriptome

| PRJNA841602 | ENA

Chara corallina

Project description:High-throughput sequencing of small RNAs in Chara corallina

| PRJNA143105 | ENA