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Salmonella Typhimurium DT104 isolates

ABSTRACT: Salmonella Typhimurium DT104 isolates

PROVIDER: PRJEB2189 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR026015_1.fastq.gz	Fastqsanger.gz
	ERR026015_2.fastq.gz	Fastqsanger.gz
	ERR026016_1.fastq.gz	Fastqsanger.gz
	ERR026016_2.fastq.gz	Fastqsanger.gz

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Transcription profiling of Salmonella enterica serovar Typhimurium definitive phage type 104 (DT104) isolates

Project description:Salmonella enterica serovar Typhimurium (S. Typhimurium) definitive phage type 104 (DT104) has caused significant morbidity and mortality in humans and animals for almost three decades. We have completed the full DNA sequence of one DT104 strain, NCTC13348 and show that the main differences between the genome of this isolate and the previously sequenced S. Typhimurium LT2 lie in integrated prophage elements and the Salmonella Genomic Island 1 encoding antibiotic resistance genes. Thirteen isolates of S. Typhimurium DT104 with different pulsed field gel electrophoresis (PFGE) profiles were analyzed by multi locus sequence typing (MLST), plasmid profiling, hybridization to a Pan-Salmonella DNA microarray and prophage-based multiplex PCR. All the isolates belonged to a single MLST type ST19. Microarray data demonstrated that the 13 DT104 isolates were remarkably conserved in gene content. The PFGE band-size differences in these isolates could be explained to a great extent by changes in prophage and plasmid content. Thus, here the nature of variation in different S. Typhimurium DT104 isolates is further defined at the genome level illustrating how this phage type is evolving over time.

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