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Extensive culturing of bladder bacteria reveals a highly connected urogenital microbiome

ABSTRACT: Bladder culture collection reveals interconnected urogenital microbiome

PROVIDER: PRJEB22112 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2092980_1.fastq.gz	Fastqsanger.gz
	ERR2092980_2.fastq.gz	Fastqsanger.gz
	ERR2092981_1.fastq.gz	Fastqsanger.gz
	ERR2092981_2.fastq.gz	Fastqsanger.gz
	ERR2092972_1.fastq.gz	Fastqsanger.gz

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Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination ofmicrodissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 bladder, urogenital sinus, and urethra. Experiment Overall Design: Bladder, urogenital sinus, and urethra regions from embryonic day 14 SMGA/EGFP animals were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

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