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NGS sequencing of plant DNA to detect botanical composition and geographic origin of honey

ABSTRACT: Application of next generation semiconductor based sequencing of a plant DNA barcoding region to detect the botanical composition and geographic origin of monofloral, polyfloral and honeydew honey

PROVIDER: PRJEB22145 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2094810.fastq.gz	Fastqsanger.gz
	ERR2094811.fastq.gz	Fastqsanger.gz
	ERR2094812.fastq.gz	Fastqsanger.gz
	ERR2094813.fastq.gz	Fastqsanger.gz
	ERR2094814.fastq.gz	Fastqsanger.gz

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Characterization of loquat (Eriobotrya japonica Lindl.) honey botanical origin according to its chitinase activity

Project description:Bees make honey from the nectar that they collect from flowers. The characteristics of honey are closely associated to original botanical species. Compare with sugars in honey, proteins are minor components but usually used as an important honey quality evaluation parameters. Flower-origin proteins could be a good marker for the authentication. However, as a minute component in honey proteome, plant origin proteins are hard to be detected in honey by regular proteomic approaches, such as gel-based techniques. In this study, Eriobotrya japonica Lindl. (loquat) nectar and its derivative monofloral honey were systematically compared, especially regarding the proteomes and enzymatic activities. Using two-dimensional electrophoresis and mass spectrometry, only bee-originated proteins were detected in loquat honey which were major royal jelly proteins and two uncharacterized proteins. Xylosidase, thaumatin, and two kinds of chitinases were detected in loquat floral nectar by the gel-based proteomic approach. To our knowledge, it is the first study to analysis nectar-originated enzymes’ activity in honey and we proposed that the zymography of chitinase is a potential marker for honey botanical origin authentication.

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