Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of transplanted pluripotent stem cells-derived human intestinal organoids upon mechanical lengthening and human jejunum tissues

ABSTRACT: RNA-seq of transplanted pluripotent stem cells-derived human intestinal organoids upon mechanical lengthening and human jejunum tissues

PROVIDER: PRJEB22611 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2119460_1.fastq.gz	Fastqsanger.gz
	ERR2119460_2.fastq.gz	Fastqsanger.gz
	ERR2119461_1.fastq.gz	Fastqsanger.gz
	ERR2119461_2.fastq.gz	Fastqsanger.gz
	ERR2119462_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 22

Similar Datasets

RNA-seq of transplanted pluripotent stem cells-derived human intestinal organoids upon mechanical lengthening and human jejunum tissues

Project description:Human Intestinal Organoids (HIOs) generated from embryonic and/or induced pluripotent stem cell lines offer an avenue to study both developmental and human specific disease states. Recently, progress has been made in scaling and maturing these inherently immature tissues through transplanting them in vivo. However, these resultant grafts best approximate fetal intestinal tissue thus limiting their utility. To induce growth and maturation of HIOs we used a nitinol spring device to mechanically induce enterogenesis of HIO in vivo. HIOs are cultured prior to implantation within the mesentery of immunodeficient mice. They are allowed to grow, vascularize, and mature before a second procedure is performed wherein a compressed nitinol spring is implanted within the lumen of the transplanted HIO (tHIO). Next Generation RNA sequencing was performed across transplanted samples as well as on human surgical samples to highlight the transcriptional similarities and differences between groups. Transcriptionally, the tHIO+S samples were more similar to human tissues than the tHIO. With these initial experiments, we concluded that the application of an intraluminal uniaxial force is a practical method to induce maturation of tHIOs in vivo without concomitant architectural disruptions. While our current system does lack certain complexities, we have demonstrated enterogenesis by means of mechanical manipulation.

2018-03-30 | E-MTAB-6017 | biostudies-arrayexpress

Peptidomics profiling of biopsies from the human jejunum before and after gastrectomy

Project description:Profiling of the human jejunum peptidome before and after total gastrectomy. The study compares the peptidome of jejunum using intact mass spectrometry and database searching. These peptides included most gut hormones including proglucagon derived peptides, PYY, GIP, Motilin somatostatin and neurotensin amongst others.

2019-02-15 | PXD011498 | Pride

ATAC-seq from jejunum (ENCSR415LEB)

Project description:ATAC-seq on human jejunum For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2022-10-16 | GSE215497 | GEO

scRNA-seq of human fetal intestinal jejunum tissue

Project description:scRNA-seq of human fetal intestinal jejunum tissue

| PRJEB50144 | ENA

Acute molecular responses to lengthening vs shortening muscle contractions

Project description:To help elucidate mechanistic differences between lengthening and shortening muscle contractions we compared changes in gene expression within 24 h of an acute bout of each type of contraction in the quadriceps. Three healthy young men performed shortening contractions with one leg while the contralateral leg performed lengthening contractions. Biopsies were taken from both legs before exercise, 3, 6, and 24 h afterwards. Expression profiling was performed using a custom-made Affymetrix MuscleChip containing probesets of approximately 3,300 known genes and ESTs expressed in skeletal muscle. We identified 49 genes differentially regulated between the lengthening and shortening contractions. Using unsupervised hierarchical clustering, we identified four distinct clusters, three of which corresponded to unique functional categories (protein synthesis, stress response/early growth, and sarcolemmal structure). The results suggest distinct molecular pathways as early as 3 h post exercise. The molecular differences might contribute to mechanisms underlying the physiological adaptations seen with training using the two modes of exercise. Keywords: Time Series Design The hypothesis of the current study was that genes involved in hypertrophy will be differentially regulated between the two types of contractions.

2007-03-27 | E-GEOD-7286 | biostudies-arrayexpress

scRNA-seq of human fetal intestinal jejunum tissue

Project description:Here, we performed single-cell RNA sequencing (scRNA-seq) of a human fetal jejunum tissue sample from 1 individual biological specimen age 40 weeks post conception. The data set is composed of cells from diverse intestinal lineages.

2022-02-01 | E-MTAB-11335 | biostudies-arrayexpress

Swine jejunum at single cell level

Project description:jejunum scRNA-seq

2024-01-01 | GSE164516 | GEO

Transplanted Human Intestinal Organoids: A Resource for Modeling Human Intestinal Development

Project description:Transplanted Human Intestinal Organoids: A Resource for Modeling Human Intestinal Development

| PRJNA942271 | ENA

Maturation and circuit integration of transplanted human cortical organoids

Project description:Maturation and circuit integration of transplanted human cortical organoids

| PRJNA788512 | ENA

Methionine supplementation and radiation in mouse jejunum

Project description:Methionine supplementation exarcerbates radiation injury in the mouse jejunum