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ChIP-Seq to investigate the opposing role of Dpf2 and Eed on differentiation of Mouse Embryonic Stem Cells via regulating Tbx3

ABSTRACT: ChIP-Seq to investigate the opposing role of Dpf2 and Eed on differentiation of Mouse Embryonic Stem Cells via regulating Tbx3

PROVIDER: PRJEB23285 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2188520.fastq.gz	Fastqsanger.gz
	ERR2188530.fastq.gz	Fastqsanger.gz
	ERR2188521.fastq.gz	Fastqsanger.gz
	ERR2188531.fastq.gz	Fastqsanger.gz
	ERR2188522.fastq.gz	Fastqsanger.gz

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Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (4C)

Project description:4C experiments of wild-type and Dpf2 -/- cells to investigate the effect of Dpf2 on the chromatin contacts of Tbx3 locus.

2018-11-30 | E-MTAB-6197 | biostudies-arrayexpress

Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (4C)

Project description:Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (4C)

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Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (ChIP-seq)

Project description:ChIP-seq experiments of 3xFlag-Dpf2, Oct4, Sox2 and several histone marks were performed in wild-type and Dpf2 -/- cells to investigate the effect of Dpf2 on the binding of those factors.

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Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (RNA-seq)

Project description:Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (RNA-seq)

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Opposing Role of Dpf2 and Eed on Differentiation of Mouse Embryonic Stem Cells via Regulating Tbx3 (RNA-seq)

Project description:RNA-seq experiments of wild-type E14 cells and Dpf2 knockout cells (OHT treated)

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The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation.

Project description:BAF complexes are composed of different subunits with varying functional and developmental roles, although many subunits have not been examined in depth. Here we show that the Baf45 subunit Dpf2 maintains pluripotency and ESC differentiation potential. Dpf2 co-occupies enhancers with Oct4, Sox2, p300, and the BAF subunit Brg1, and deleting Dpf2 perturbs ESC self-renewal, induces repression of Tbx3, and impairs mesendodermal differentiation without dramatically altering Brg1 localization. Mesendodermal differentiation can be rescued by restoring Tbx3 expression, whose distal enhancer is positively regulated by Dpf2-dependent H3K27ac maintenance and recruitment of pluripotency TFs and Brg1. In contrast, the PRC2 subunit Eed binds an intragenic Tbx3 enhancer to oppose Dpf2-dependent Tbx3 expression and mesendodermal differentiation. The PRC2 subunit Ezh2 likewise opposes Dpf2-dependent differentiation through a distinct mechanism involving Nanog repression. Together, these findings delineate distinct mechanistic roles for specific BAF and PRC2 subunits during ESC differentiation.

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Dpf2 interactome in mouse embryonic stem cells

Project description:Dpf2 is a subunit of the BAF/pBAF chromatin remodelling complex. We have used cross-linking affinity purification-mass spectrometry to explore Dpf2 protein interactions in mouse embryonic stem cells.

2019-11-13 | PXD011806 | Pride

Hierarchical deployment of Tbx3 dictating the identity of hypothalamic Tac2/Kiss1 neurons

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Project description:Tbx3 regulates liver development in the mouse, but it's role in human liver development is unclear. We generated TBX3 knockout pluripotent stem cell lines and found that liver differentiation is also impaired in humans. Interestingly we noted expression of pancreas-specific genes during the liver differentiation in the TBX3 knockout line. To further investigate this phenomenon, we performed RNA-seq on cells at the hepatoblast stage of the liver differentiation. These studies demonstrated that the loss of TBX3 enhances pancreatic-gene signature, implicating TBX3 as a regulator of fate decisions.

2021-10-14 | GSE180526 | GEO

Loss of TBX3 enhances pancreatic progenitor generation from human pluripotent stem cells [Pancreas RNA-seq]

Project description:Tbx3 regulates liver development in the mouse, but it's role in human liver development is unclear. We generated TBX3 knockout pluripotent stem cell lines and found that liver differentiation is also impaired in humans. Interestingly we noted expression of pancreas-specific genes during the liver differentiation in the TBX3 knockout line.To further investigate this phenomenon, we performed RNA-seq on cells from two stages of the pancreatic differentation. These studies demonstrated that the loss of TBX3 enhances pancreatic-gene signature, implicating TBX3 as a regulator of fate decisions.

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