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STEC O157 isolates from Australia

ABSTRACT: STEC O157 isolates from Australia

PROVIDER: PRJEB23370 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2192280_1.fastq.gz	Fastqsanger.gz
	ERR2192280_2.fastq.gz	Fastqsanger.gz
	ERR2192281_1.fastq.gz	Fastqsanger.gz
	ERR2192281_2.fastq.gz	Fastqsanger.gz
	ERR2192282_1.fastq.gz	Fastqsanger.gz

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Project description:We compared the transcriptional profiles of 12 E. coli O157:H7 isolates grown to stationary phase in LB broth. These isolates possess the same two enzyme PFGE profile and are related temporally or geographically to the above outbreak. These E. coli O157:H7 isolates included three clinical isolates, five isolates from separate bags of spinach, and single isolates from pasture soil, river water, cow feces, and a feral pig. Twelve condition experiment, 12 E. coli O157:H7 isolates. Two biological replicates for isolates RM6067, RM6069, RM6101, RM6102, RM6103, RM6149, RM6655, RM6658, RM9992, RM9997, RM9998 and RM10002 independently grown to stationary phase in LB at 37°C and harvested. One replicate per array. A type 2 gene expression experimental design was used, with fluorescently labeled genomic DNA as a reference channel in each experiment as described by Lucchini, S., et al. 2005. Infect Immun 73:88-102.

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