Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

STEC O157 isolates from Australia

ABSTRACT: STEC O157 isolates from Australia

PROVIDER: PRJEB23370 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2192280_1.fastq.gz	Fastqsanger.gz
	ERR2192280_2.fastq.gz	Fastqsanger.gz
	ERR2192281_1.fastq.gz	Fastqsanger.gz
	ERR2192281_2.fastq.gz	Fastqsanger.gz
	ERR2192282_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 26

Similar Datasets

SF O157 STEC outbreak isolate

Project description:SF O157 STEC outbreak isolate

| PRJEB20962 | ENA

French outbreak of STEC O157:H7

Project description:French outbreak of STEC O157:H7

| PRJEB52802 | ENA

Escherichia coli O157:H7 Isolates Related to the 2006 Spinach-associated Outbreak Exhibit Distinct Transcriptional Profiles

2011-11-01 | GSE33131 | GEO

Comparative Transcriptomics Analysis of Shiga Toxin Producing Escherichia coli O157:H7 Grown in Bovine Rectoanal Junction Cells and Human Colonic Epithelial Cells

Project description:Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a notorious foodborne pathogen capable of causing severe gastrointestinal infections in humans. The bovine rectoanal junction (RAJ) has been identified as a primary reservoir of STEC O157:H7, playing a critical role in its transmission to humans through contaminated food sources. Despite the relevance of this host-pathogen interaction, the molecular mechanisms behind the adaptation of STEC O157:H7 in the bovine RAJ and its subsequent infection of human colonic epithelial cells remain largely unexplored. This study aimed to unravel the intricate dynamics of STEC O157:H7 in two distinct host environments: bovine RAJ squamous epithelial (RSE) cells and human colonic epithelial cells. Comparative transcriptomics analysis was employed to investigate the differential gene expression profiles of STEC O157:H7 during its interaction with these cell types. The bacterial cells were cultured under controlled conditions to simulate the microenvironments of both bovine RAJ and human colonic epithelial cells. Using high-throughput RNA sequencing, we identified key bacterial genes and regulatory pathways that are significantly modulated in response to each specific host environment. Our findings reveal distinct expression patterns of virulence factors, adhesion proteins, and stress response genes in STEC O157:H7 grown in bovine RAJ cells as opposed to human colonic epithelial cells. Additionally, the comparative analysis highlights the potential role of certain genes in host adaptation and tissue-specific pathogenicity. Furthermore, this study sheds light on the potential factors contributing to the survival and persistence of STEC O157:H7 in the bovine reservoir and its ability to colonize and cause disease in humans.

2023-11-09 | GSE240423 | GEO

Escherichia coli O157:H7 Isolates Related to the 2006 Spinach-associated Outbreak Exhibit Distinct Transcriptional Profiles

Project description:We compared the transcriptional profiles of 12 E. coli O157:H7 isolates grown to stationary phase in LB broth. These isolates possess the same two enzyme PFGE profile and are related temporally or geographically to the above outbreak. These E. coli O157:H7 isolates included three clinical isolates, five isolates from separate bags of spinach, and single isolates from pasture soil, river water, cow feces, and a feral pig. Twelve condition experiment, 12 E. coli O157:H7 isolates. Two biological replicates for isolates RM6067, RM6069, RM6101, RM6102, RM6103, RM6149, RM6655, RM6658, RM9992, RM9997, RM9998 and RM10002 independently grown to stationary phase in LB at 37°C and harvested. One replicate per array. A type 2 gene expression experimental design was used, with fluorescently labeled genomic DNA as a reference channel in each experiment as described by Lucchini, S., et al. 2005. Infect Immun 73:88-102.

2011-11-01 | E-GEOD-33131 | biostudies-arrayexpress

Escherichia coli O157

Project description:STEC O157 intrafamily infection

| PRJDB10137 | ENA

Escherichia coli O157

Project description:STEC O157 from animals

| PRJDB11700 | ENA

STEC Outbreak

Project description:Largest STEC Outbreak reported to date in Australia

| PRJEB15317 | ENA

E. coli O157:H7 phage type 21/28 & 32 CGH analysis

Project description:Six isolates of PT21/28 and six of PT32 were analysed by CGH using UBECarray3 microarrays (containing probes for E. coli K-12 str. MG1655 and O157:H7 str. EDL933 and Sakai) to define genotypic differences between phage types. gDNA from E.coli O157 str. Sakai was hybridised to all arrays to provide a universal control channel on all arrays.

2012-04-21 | GSE28838 | GEO

RNA SEQ STEC FHI96 Type III mod

Project description:Comparison of RNA expression profiles from STEC strain FHI96 expressing methyltransferase mod (ON; 52reps) or not (OFF; 51reps).

2024-06-30 | GSE129787 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data