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Transcriptome profiling by high throughput sequencing of HeLa cells depleted of MATR3, PTBP1/2 individually or in combination by siRNA.

ABSTRACT: Transcriptome profiling by high throughput sequencing of HeLa cells depleted of MATR3, PTBP1/2 individually or in combination by siRNA.

PROVIDER: PRJEB23439 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2194420.fastq.gz	Fastqsanger.gz
	ERR2194430.fastq.gz	Fastqsanger.gz
	ERR2194440.fastq.gz	Fastqsanger.gz
	ERR2194450.fastq.gz	Fastqsanger.gz
	ERR2194421.fastq.gz	Fastqsanger.gz

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Transcriptome profiling by high throughput sequencing of HeLa cells depleted of MATR3, PTBP1/2 individually or in combination by siRNA.

Project description:We performed RNA-seq experiments on three biological replicates of HeLa cells depleted of MATR3, PTBP1/2, controls, or combined depletion of MATR3/PTBP1/2. Cells were fractionated into cytoplasmic and nuclear RNA. Library preparation was done with the TruSeq stranded RNAseq library kit (Illumina) according to manufacturer’s recommendations; RNA was depleted of rRNA using the RiboZero kit (Epicentre). All libraries were sequenced on Illumina HiSeq2 machines in a single-end manner with a read length of 100 nt.

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