Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Wild-type Lister 427 Bloodstream-form polysome profiles with ZC3H32 knock-down

ABSTRACT: Wild-type Lister 427 Bloodstream-form polysome profiles with ZC3H32 knock-down

PROVIDER: PRJEB23630 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Wild-type Lister 427 Bloodstream-form polysome profiles with ZC3H32 knock-down

Project description:Ran from polysomes of Lister 427 bloodstream forms, wild-type and a cell line with a double knockout and inducible ZC3H32, grown without tetracycline.

2017-11-24 | E-MTAB-4558 | biostudies-arrayexpress

Trypanosoma brucei strain:Lister 427

Project description:Trypanosoma brucei strain:Lister 427 Raw sequence reads

| PRJNA526543 | ENA

Trypanosoma brucei brucei strain:Lister 427 | isolate:PN221

Project description:Trypanosoma brucei brucei Lister 427 isolate

| PRJNA991312 | ENA

Trypanosoma brucei brucei strain:Lister 427

Project description:Trypanosoma brucei brucei strain:Lister 427 Raw sequence reads

| PRJNA296500 | ENA

Trypanosoma brucei brucei strain:Lister 427

Project description:Trypanosoma brucei brucei strain:Lister 427 Targeted Locus (Loci)

| PRJNA247996 | ENA

Trypanosoma brucei brucei strain:Lister 427

Project description:Trypanosoma brucei brucei strain:Lister 427 DNA or RNA sequencing

| PRJNA934938 | ENA

Trypanosoma brucei brucei strain:Lister 427

Project description:Trypanosoma brucei brucei strain:Lister 427 Transcriptome or Gene expression

| PRJNA255753 | ENA

Trypanosoma brucei brucei strain:Lister 427

Project description:Trypanosoma brucei brucei strain:Lister 427 Transcriptome or Gene expression

| PRJNA248941 | ENA

Measurement of variant surface glycoprotein mRNA using RNAseq

Project description:Trypanosoma brucei Lister 427 bloodstream forms were cultured in HMI-11 medium. Total RNA was prepared using Qiagen RNAeasy kits for single sample RNAseq to estimate VSG mRNA abundance (and not to reconstruct the transcriptome). The cDNA libraries were prepared and sequenced at the Beijing Genomics Institute (Shenzhen, China). Polyadenylated RNA was purified from total RNA, converted to cDNA using random hexamer primers sheared and size selected for fragments ~200 bp in length using the Illumina TruSeq RNA Sample Preparation Kit v2. RNAseq of the resulting libraries was used for the determination of transcript abundances. Sequencing was performed on an Illumina Hiseq 2000 (Illumina, CA) platform and 90 base paired end reads obtained. Four samples were analysed: 1. Trypanosoma brucei Lister 427 expressing VSG2 2. Trypanosoma brucei Lister 427 expressing VSG6 3. Trypanosoma brucei Lister 427 expressing VSG6 and a VSG2 transgender located in the active bloodstream expression site 28 days after electroporation 4. Trypanosoma brucei Lister 427 expressing VSG6 and a VSG2 transgender located in the active bloodstream expression site 44 days after electroporation.

2020-06-03 | E-MTAB-9122 | biostudies-arrayexpress

Trypanosoma brucei brucei strain:Lister 427 | isolate:PN221

Project description:Trypanosoma brucei brucei Lister 427 isolate, haplotype "B"

| PRJNA991301 | ENA