Project description:We performed RNA-seq analysis of the root transcriptional response to Fusarium oxysporum f.sp. vasinfectum (FOV) race 4 (FOV4) infection in Gossypium barbadense, also known as Pima cotton. Susceptible Gossypium barbadense inbred lines Pima S-7 (PI 560140) and Pima 3-79 susceptible to Fusarium wilt [Fusarium oxysporum f.sp. vasinfectum (FOV)] race 4 (FOV4), and Pima S-6 (PI 608346) which is resistant to FOV4 infection, were used for the preparation of cDNA libraries and further RNA-seq analyses. An isolate of FOV4 (FOV CA-14) from a naturally infested field in Fresno County in the San Joaquin Valley, California was used in this study.

2023-11-30 | GSE233711 | GEO

ddEupLath

Project description:Euphorbia lathyris

| PRJEB65676 | ENA

Euphorbia factor L(8): a diterpenoid from the seeds of Euphorbia lathyris.

Project description:THE TITLE COMPOUND [SYSTEMATIC NAME: (2S*,3S*,4R*,5R*,9S*,11S*,15R*)-5,15-diacet-oxy-3-nicotino-yloxy-14-oxolathyra-6(17),12(E)-diene], C(30)H(37)NO(7), was isolated from the seeds of Euphorbia lathyris. The tricyclic diterpenoid molecule contains an 11-membered ring, a five-membered ring exhibiting an envelope conformation and a three-membered ring. The 11-membered ring is cis-fused with the three-membered ring and trans-fused with the five-membered ring.

| S-EPMC2915372 | biostudies-literature

Fusarium oxysporum f.sp. radicis-lycopersici induces distinct transcriptome reprogramming in resistant and susceptible isogenic tomato lines

Project description:Background: Fusarium oxysporum f.sp. radicis-lycopersici (FORL) is one of the most destructive necrotrophic pathogen of tomato, causing important field and greenhouse yield losses. Despite the relevant economic impact, little is known about the molecular mechanisms regulating the Fusarium oxysporum f.sp. radicis-lycopersici resistance in tomato. Results: A transcriptomic experiment was carried out in order to investigate major mechanisms of FORL response in resistant and susceptible isogenic tomato lines. The microarray analysis at 15 DPI reveals a distinct gene expression pattern between the two genotypes in the inoculated vs not inoculated conditions, furthermore confirmed by a q-PCR experiment. A model of plant response for both compatible and incompatible reaction was proposed. In particular, in the incompatible interaction an activation of defense genes related to secondary metabolites production and tryptophan metabolism was observed. Moreover, the cell osmotic potential maintenance after the FORL roots challenging was mediated by a dehydration induced protein. As for the compatible interaction the activation of an oxidative burst mediated by Peroxidases and a Cytochrome monooxigenase, conducedthe cells to degeneration and necrosis. Conclusions: Our work allowed a comprehensive understanding of the molecular basis of the tomato-FORL interaction. Result obtained emphasizes a different transcriptional reaction between the resistant and the susceptible genotype to FORL challenging. Our findings could lead to the improvement of strategies of this disease control.

2017-02-09 | GSE71393 | GEO

Dataset of banana transcriptome in response to endophyte bacteria inoculation prior to Fusarium oxysporum f.sp. cubense tropical race 4 (Foc TR4) infection

Project description:We report the first data of RNA sequencing of banana Musa acuminata cv. Pisang ambon kuning (AAA group) inoculated by two different endophyte bacteria named Stenothropomonas nitritireducens (BR-49) and Kocuria rhizophila (SL-08), respectively, prior to Fusarium oxysprorum f.sp. cubense tropical race 4 (Foc TR4).

2020-01-04 | GSE143006 | GEO

Distinct triterpene synthases in the laticifers of Euphorbia lathyris.

Project description:Euphorbia lathyris was proposed about fifty years ago as a potential agroenergetic crop. The tremendous amounts of triterpenes present in its latex has driven investigations for transforming this particular biological fluid into an industrial hydrocarbon source. The huge accumulation of terpenes in the latex of many plant species represent a challenging question regarding cellular homeostasis. In fact, the enzymes, the mechanisms and the controllers that tune the amount of products accumulated in specialized compartments (to fulfill ecological roles) or deposited at important sites (as essential factors) are not known. Here, we have isolated oxidosqualene cyclases highly expressed in the latex of Euphorbia lathyris. This triterpene biosynthetic machinery is made of distinct paralogous enzymes responsible for the massive accumulation of steroidal and non-steroidal tetracyclic triterpenes. More than eighty years after the isolation of butyrospermol from shea butter (Heilbronn IM, Moffet GL, and Spring FS J. Chem. Soc. 1934, 1583), a butyrospermol synthase is characterized in this work using yeast and in folia heterologous expression assays.

| S-EPMC6423090 | biostudies-literature

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